DNA-Bioprotective Effects of Lactic Acid Bacteria against Aflatoxin B1

Lactic acid bacteria commonly named as probiotics have a broad range of utilization area in human diet and food industry. Besides these known properties of probiotics, in recent years many researchers have focused on their anti-genotoxic called DNA-bioprotective effects. Human gets exposure with gen...

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Bibliographic Details
Published in:Current research in nutrition and food science 2016-10, Vol.4 (Special-Issue-October), p.87-91
Main Authors: Kurhan, Şebnem, Çakir, Ibrahim
Format: Article
Language:English
Subjects:
Adenocarcinoma
Aflatoxin B1
Aflatoxins
Aromatic compounds
Bacteria
Bioassays
By products
Carcinogens
Colon
Comet assay
Cytotoxicity
Damage detection
Deoxyribonucleic acid
Diet
DNA
Exposure
Food
Food industry
Food processing industry
Genotoxicity
Hydrogen peroxide
Lactic acid
Lactic acid bacteria
Lactobacilli
Lactobacillus plantarum
Mammals
Metabolic activation
Metabolic rate
Microorganisms
Mycotoxins
Polycyclic aromatic hydrocarbons
Probiotics
Toxicity
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Summary:Lactic acid bacteria commonly named as probiotics have a broad range of utilization area in human diet and food industry. Besides these known properties of probiotics, in recent years many researchers have focused on their anti-genotoxic called DNA-bioprotective effects. Human gets exposure with genotoxins such as mycotoxins, polycyclic aromatic hydrocarbons, and n-nitroso-compounds through diet and environmental contaminations. Aflatoxin B1 is mycotoxin which was reported one of the most potent hepato-carcinogen and its exposure stems from human diet. In this study, we aimed to investigate DNA-bioprotective effect of Lactobacillus plantarum on human colon adenocarcinoma (Caco-2) cells against Aflatoxin B1 (AFB1) with comet assay without metabolic activation. The results showed that DNA-bioprotective effect of L. plantarum did reduce the AFB1’s genotoxic effect on colon adenocarcinoma (Caco-2) cells. Positive control (50 μM H2O2 applied) and high dose (>10ppm) AFB1 applied cells have the same comet tail appearance. Only visual scoring is performed. Besides negative control cells (Only PBS) and Lactobacillus plantarum+AFB1 mixture showed the same manner with each other; no comet tail detected. These results clearly indicate that L. plantarum is capable of reduce AFB1 safely without producing any by-products.
ISSN:2347-467X
2322-0007
DOI:10.12944/CRNFSJ.4.Special-Issue-October.11