Recoverability of Listeria monocytogenes after coculture with Tetrahymena pyriformis

Bactivory by protozoa is a major factor that limits the number of bacteria in nature and may control the presence of Listeria monocytogenes. The effectiveness of Tetrahymena pyriformis destruction of L. monocytogenes was measured. Within 1 hr, 35–40 T. pyriformis cells ingested an average of 1,219 C...

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Published in:Journal of food safety 2020-06, Vol.40 (3), p.n/a
Main Authors: Meinersmann, Richard J., Berrang, Mark E., Rigsby, Luanne L.
Format: Article
Language:English
Subjects:
Bacteria
Cell culture
Food irradiation
Food processing
Food safety
Gentamicin
Listeria
Listeria monocytogenes
Protozoa
Recoverability
Swimming
Tetrahymena pyriformis
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description Bactivory by protozoa is a major factor that limits the number of bacteria in nature and may control the presence of Listeria monocytogenes. The effectiveness of Tetrahymena pyriformis destruction of L. monocytogenes was measured. Within 1 hr, 35–40 T. pyriformis cells ingested an average of 1,219 CFU of L. monocytogenes. Gentamicin was then added to kill un‐ingested Listeria. In 24 hr, the recoverable bacteria were reduced at an exponential rate to undetectable levels (
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The effectiveness of Tetrahymena pyriformis destruction of L. monocytogenes was measured. Within 1 hr, 35–40 T. pyriformis cells ingested an average of 1,219 CFU of L. monocytogenes. Gentamicin was then added to kill un‐ingested Listeria. In 24 hr, the recoverable bacteria were reduced at an exponential rate to undetectable levels (&lt;1 per culture). A genetically diverse set of L. monocytogenes cultures all reduced Listeria recovery by the same degree. In assays without addition of gentamicin, numbers of attached L. monocytogenes cells were lessened from an average of log 6.5 CFU/2 ml culture to log 4.7 CFU/2 ml culture. T. pyriformis was capable of lowering numbers of both free‐swimming and attached L. monocytogenes. This technology may have applications to control L. monocytogenes in food processing environments. Assays to evaluate killing of Listeria monocytogenes by the protozoan Tetrahymena pyriformis were developed. Listeria were killed at an exponential rate for the first 4 hr of coculture with Tetrahymena. Nineteen diverse strains of Listeria were equally susceptible to killing by Tetrahymena. 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source Wiley-Blackwell Read & Publish Collection
subjects Bacteria
Cell culture
Food irradiation
Food processing
Food safety
Gentamicin
Listeria
Listeria monocytogenes
Protozoa
Recoverability
Swimming
Tetrahymena pyriformis
title Recoverability of Listeria monocytogenes after coculture with Tetrahymena pyriformis
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