Emergence of vancomycin-resistant Enterococcus faecium ST1421 lacking the pstS gene in Korea

• Published in: European journal of clinical microbiology & infectious diseases 2020-07, Vol.39 (7), p.1349-1356
• Main Authors: Kim, Hye Mee, Chung, Doo Ryeon, Cho, Sun Young, Huh, Kyungmin, Kang, Cheol-In, Peck, Kyong Ran
• Format: Article
• Language: English
• Subjects: Alleles; Antibiotic resistance; Antibiotics; Bacterial Proteins - genetics; Biomedical and Life Sciences; Biomedicine; Clinical isolates; DNA Transposable Elements; Electrophoresis; Emergence; Enterococcus faecium; Enterococcus faecium - classification; Enterococcus faecium - genetics; Enterococcus faecium - isolation & purification; Epidemiology; Gel electrophoresis; Gene Deletion; Gene sequencing; Genes, Essential - genetics; Genome, Bacterial - genetics; Genomes; Gram-Positive Bacterial Infections - epidemiology; Gram-Positive Bacterial Infections - microbiology; Humans; Insertion sequences; Internal Medicine; Medical Microbiology; Multilocus Sequence Typing; Original Article; Pattern analysis; Phosphate-Binding Proteins - genetics; Phylogeny; Population structure; Prevalence; PstS gene; Pulsed-field gel electrophoresis; Rectum; Republic of Korea - epidemiology; Transposase; Vancomycin; Vancomycin-Resistant Enterococci - drug effects; Vancomycin-Resistant Enterococci - genetics; Vancomycin-Resistant Enterococci - isolation & purification; Whole genome sequencing
• ISSN: 0934-9723; 1435-4373
• DOI: 10.1007/s10096-020-03853-4

Although multilocus sequence typing (MLST) has been used to study molecular epidemiology and to explore the population structure of Enterococcus faecium , vancomycin-resistant E. faecium (VREF) strains lacking the pstS gene that were non-typable using conventional MLST methods were reported recently. We found nationwide emergence of VREF isolates lacking pstS in Korea and hereby report the molecular characteristics of these isolates. Forty-six VREF isolates lacking the pstS gene were identified among 300 VREF rectal isolates collected from hospitalized patients between 2014 and 2015. MLST was performed and clonal relatedness was determined by pulsed-field gel electrophoresis (PFGE). Four VREF ST1421 isolates were whole-genome sequenced. Among the VREF rectal isolates lacking pstS , 98% were classified as ST1421, which has identical allelic profiles to ST17 for all housekeeping genes except pstS . PFGE pattern analyses revealed 32 pulsotypes. All isolates harbored Tn 1546 components with various transposase and insertion sequences. The whole-genome sequencing of four VREF ST1421 isolates showed that the pstS gene region was deleted at various locations with considerable inversion. The pstS gene was also depleted in 12.1% of 33 VREF clinical isolates in 2006–2007 and in 11.8% of 59 clinical isolates in 2012–2013. VREF ST1421 strains lacking the pstS gene have emerged in Korea. The emergence and spread of pstS -deleted VREF strains pose a serious challenge for epidemiological investigation. Alternative molecular typing methods to MLST will be increasingly necessary.