Validation of a Western immunoblotting procedure for bovine PrPSc detection and its use as a rapid surveillance method for the diagnosis of bovine spongiform encephalopathy (BSE)

In this report we document the results of several independent studies testing the sensitivity, specificity and reliability of the Prionics Western blotting (PWB) procedure to detect bovine and ovine disease-specific, protease-resistant prion protein (PrPSc). Validation of the technique was obtained...

Full description

Saved in:
Bibliographic Details
Published in:Acta neuropathologica 1999-11, Vol.98 (5), p.437-443
Main Authors: SCHALLER, O, FATZER, R, OESCH, B, MOSER, M, STACK, M, CLARK, J, COOLEY, W, BIFFIGER, K, EGLI, S, DOHERR, M, VANDEVELDE, M, HEIM, D
Format: Article
Language:English
Subjects:
Abattoirs
Biological and medical sciences
Bovine spongiform encephalopathy
Cattle
Culling
Diagnosis
Immunoblotting
Immunohistochemistry
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
Neurological diseases
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Prion protein
Sensitivity analysis
Surveillance
Western blotting
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this report we document the results of several independent studies testing the sensitivity, specificity and reliability of the Prionics Western blotting (PWB) procedure to detect bovine and ovine disease-specific, protease-resistant prion protein (PrPSc). Validation of the technique was obtained by blind analysis of samples from cattle affected with bovine spongiform encephalopathy (BSE), clinically normal animals or cattle with neurological diseases unrelated to BSE. Overall, very high sensitivity, specificity and reliability was observed. It became clear that sampling of the correct brain region and the method used for protein extraction are important factors for correct diagnosis. Furthermore, we tested the usefulness of the PWB technique as an instrument for surveillance purposes. We analyzed animals from a culling scheme as well as older animals from abattoirs to determine the number of subclinical BSE cases detectable by histopathological examination, immunohistochemistry for PrPSc and PWB. In both studies, BSE-affected animals with no overt clinical symptoms were detected. These results demonstrate the usefulness of the PWB procedure in surveillance systems serving as a rapid diagnostic tool to identify animals subclinically infected with BSE.
ISSN:0001-6322
1432-0533
DOI:10.1007/s004010051106