Tumor necrosis factor-α expression in muscles of polymyositis and dermatomyositis

We evaluated the expression of tumor necrosis factor-alpha (TNF-alpha) mRNA in muscle biopsy specimens from patients with polymyositis (PM) and dermatomyositis (DM) to clarify its role in the pathogenesis of PM and DM. We performed non-radioactive in situ hybridization studies for TNF-alpha combined...

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Bibliographic Details
Published in:Acta neuropathologica 2000-05, Vol.99 (5), p.585-588
Main Authors: KURU, S, INUKAI, A, YIDENG LIANG, DOYU, M, TAKANO, A, SOBUE, G
Format: Article
Language:English
Subjects:
Aged
Biological and medical sciences
Biopsy
CD8 antigen
Degeneration
Dermatomyositis
Dermatomyositis - pathology
Dermatomyositis - physiopathology
Female
Gene Expression
Humans
Hybridization
Immunohistochemistry
In Situ Hybridization
Lymphocytes
Macrophages
Male
Medical sciences
Middle Aged
mRNA
Muscle Fibers, Skeletal - pathology
Muscle, Skeletal - pathology
Muscle, Skeletal - physiology
Muscles
Necrosis
Polymyositis
RNA, Messenger - analysis
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
Tumor Necrosis Factor-alpha - genetics
Tumor necrosis factor-TNF
Tumor necrosis factor-α
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Summary:We evaluated the expression of tumor necrosis factor-alpha (TNF-alpha) mRNA in muscle biopsy specimens from patients with polymyositis (PM) and dermatomyositis (DM) to clarify its role in the pathogenesis of PM and DM. We performed non-radioactive in situ hybridization studies for TNF-alpha combined with immunohistochemistry for cell type-specific markers on muscles from ten PM and five DM patients. TNF-alpha-positive infiltrating cells present in the endomysium and perimysium were found in all PM and DM muscles. The frequency of TNF-alpha-positive cells against total infiltrating cells was similar among PM and DM (27.1 +/- 7.4% in PM and 28.5 +/- 13.6% in DM). However, TNF-alpha/CD8-positive lymphocytes and TNF-alpha-positive macrophages invading the non-necrotic muscle fiber were observed only in PM but not in DM. TNF-alpha was more highly expressed in PM and DM than was previously thought, and it was suggested that TNF-alpha plays a role in muscle fiber degeneration in PM.
ISSN:0001-6322
1432-0533
DOI:10.1007/s004010051165