Rapid detection of antibiotic resistance genes in lactic acid bacteria using PMMA-based microreactor arrays

The emergence of lactic acid bacteria (LABs) resistant to existing antimicrobial drugs is a growing health crisis. To decrease the overuse of antibiotics, molecular diagnostic systems that can rapidly determine the presence of antibiotic resistance (AR) genes in LABs from yogurt samples are needed....

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2020-07, Vol.104 (14), p.6375-6383
Main Authors: Jin, Zengjun, Ding, Guotao, Yang, Guoxing, Li, Guiying, Zhang, Wei, Yang, Lixin, Li, Weihao
Format: Article
Language:English
Subjects:
Acid resistance
Acids
Amplification
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Antibiotics
Antimicrobial agents
Bacteria
Biomedical and Life Sciences
Biotechnology
Chemical properties
Deoxyribonucleic acid
Diagnosis
Diagnostic systems
DNA
DNA microarrays
Drug resistance
Drug resistance in microorganisms
Drug Resistance, Bacterial - genetics
Food Microbiology
Genes
Genes, Bacterial
Genetic aspects
Lactic acid
Lactic acid bacteria
Lactobacillales - drug effects
Lactobacillales - genetics
Lactobacillales - isolation & purification
Lactobacillus
Life Sciences
Methods and Protocols
Microbial Genetics and Genomics
Microbial Sensitivity Tests
Microbiology
Microfluidics - instrumentation
Microfluidics - methods
Multiplexing
Nucleic Acid Amplification Techniques
Nucleic acids
Polymethyl Methacrylate
Polymethylmethacrylate
Sensitivity and Specificity
Streptomycin
Vancomycin
Yogurt
Yogurt - microbiology
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Summary:The emergence of lactic acid bacteria (LABs) resistant to existing antimicrobial drugs is a growing health crisis. To decrease the overuse of antibiotics, molecular diagnostic systems that can rapidly determine the presence of antibiotic resistance (AR) genes in LABs from yogurt samples are needed. This paper describes a fully integrated, miniaturized plastic chip and closed-tube detection chemistry that performs multiplex nucleic acid amplification. High-throughput identification of AR genes was achieved through this approach, and six AR genes were analyzed simultaneously in
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-020-10699-5