In silico characterisation and homology modelling of a pathogenesis-related protein from Saccharum arundinaceum

Pathogenesis related 10 (PR10) proteins are small, cytosolic proteins sharing a similar three-dimensional structure and diverse functions. The present study aimed to characterise in silico the PR10.1 protein from Saccharum arundinaceum (SaPR10.1), a wild relative of sugarcane. SaPR10.1 is a hydrophi...

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Bibliographic Details
Published in:Archiv für Phytopathologie und Pflanzenschutz 2020-03, Vol.53 (5-6), p.199-216
Main Authors: Mohan, Chakravarthi, Santos Júnior, Célio Dias, Chandra, Swarnendu
Format: Article
Language:English
Subjects:
Depletion
Erianthus
Homology
in silico
Molecular docking
Pathogenesis
pathogenesis-related protein
Phylogeny
PR10
Protein structure
Proteins
Ribonuclease
Saccharum arundinaceum
Secondary structure
Sorghum
Sugarcane
trans-zeatin
Zeatin
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Summary:Pathogenesis related 10 (PR10) proteins are small, cytosolic proteins sharing a similar three-dimensional structure and diverse functions. The present study aimed to characterise in silico the PR10.1 protein from Saccharum arundinaceum (SaPR10.1), a wild relative of sugarcane. SaPR10.1 is a hydrophilic small acidic protein (17 kDa) that lacks the P-loop motif presumed to be involved in the RNase activity for some PR-10 proteins. Predictions of a rich secondary structure present in SaPR10.1 led us to a protein model that suggests SaPR10.1 can bind to more than one molecule of trans-zeatin using molecular docking. This finding suggests that SaPR10.1, differently from the orthologs, can be involved in more efficient depletion of free trans-zeatin. This idea is reinforced by the phylogenetic analysis, which showed both variants SaPR10 and SaPR10.1 closely related to other monocot species like sorghum, maize, and broomcorn millet and not to wheat.
ISSN:0323-5408
1477-2906
DOI:10.1080/03235408.2020.1736739