Loading…

Symbiotic, phenotypic and genotypic characterization of Bradyrhizobium sp. nodulating Spartium junceum L. from Bejaia, northeastern Algeria

Twenty-three bacterial strains were isolated from root nodules of Spartium junceum L., growing wild in four distinct geographic locations in Bejaia city (Northeastern Algeria). They were analyzed based on their symbiotic effectiveness, phenotypic properties and restriction fragment length polymorphi...

Full description

Saved in:
Bibliographic Details
Published in:Symbiosis (Philadelphia, Pa.) Pa.), 2020-05, Vol.81 (1), p.25-37
Main Authors: Ramdani, Nacer, Belhadi, Djellali, Kaci, Yahia, Benallaoua, Said
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c270t-5409fe708e370e4002eaccdf72cb0c08f9b53a786243997c7e683afaab1025c23
container_end_page 37
container_issue 1
container_start_page 25
container_title Symbiosis (Philadelphia, Pa.)
container_volume 81
creator Ramdani, Nacer
Belhadi, Djellali
Kaci, Yahia
Benallaoua, Said
description Twenty-three bacterial strains were isolated from root nodules of Spartium junceum L., growing wild in four distinct geographic locations in Bejaia city (Northeastern Algeria). They were analyzed based on their symbiotic effectiveness, phenotypic properties and restriction fragment length polymorphism of the 16S rRNA gene. In addition, sequence analyses of the ribosomal gene, protein-coding housekeeping genes glnII and recA , and symbiotic gene ( nodC ) were used to clarifying their taxonomic and phylogenetic position. Plant infection tests revealed that all strains were able to form nodules on their original host plant, but with variable nodulation ability and symbiotic effectiveness. All strains were slow-growing, alkali-producing rhizobia in YEMA medium. According to phenotypic test results, the strains were grouped in four main clusters in a UPGMA dendrogram, while they were discriminated into 12 distinct genotypes by 16S rDNA-RFLP. Phylogenetic analyses of 16S rRNA genes and two concatenated housekeeping genes, glnII and recA , distributed the representative strains from each RFLP genotypes into two major clades within Bradyrhizobium genus. Some of these strains were closely related to B. retamae and B. japonicum whereas others represented four novel genospecies. Based on analysis of the symbiotic gene sequence ( nodC ), all strains were placed in the symbiovar retamae cluster except for SjBA12 which possibly constitutes a new symbiovar within the genus Bradyrhizobium .
doi_str_mv 10.1007/s13199-020-00679-8
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2419205660</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2419205660</sourcerecordid><originalsourceid>FETCH-LOGICAL-c270t-5409fe708e370e4002eaccdf72cb0c08f9b53a786243997c7e683afaab1025c23</originalsourceid><addsrcrecordid>eNp9kM1OwzAQhC0EEuXnBThZ4krK2k7s5AiIP6kSB-BsbVynddXawU4O5RV4aQxFcOM02tU3s9oh5IzBlAGoy8QEa5oCOBQAUjVFvUcmrFZ1oaSs9skEhCiLirHykByltAIQTAo5IR_P203rwuDMBe2X1odh2ztD0c_p4ncyS4xoBhvdOw4ueBo6eh1xvo1L9x5aN25o6qfUh_m4zoBf0Oce4_C1X43e2KyzKe1i2NBru0KHF5mNw9JiyqGeXq0XORtPyEGH62RPf_SYvN7dvtw8FLOn-8ebq1lhuIKhqEpoOqugtkKBLQG4RWPmneKmBQN117SVQFVLXoqmUUZZWQvsEFsGvDJcHJPzXW4fw9to06BXYYw-n9S8ZA2HSkrIFN9RJoaUou10H90G41Yz0F-l613pOpeuv0vXdTaJnSll2Oev_qL_cX0Ct-yHiA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2419205660</pqid></control><display><type>article</type><title>Symbiotic, phenotypic and genotypic characterization of Bradyrhizobium sp. nodulating Spartium junceum L. from Bejaia, northeastern Algeria</title><source>Springer Nature</source><creator>Ramdani, Nacer ; Belhadi, Djellali ; Kaci, Yahia ; Benallaoua, Said</creator><creatorcontrib>Ramdani, Nacer ; Belhadi, Djellali ; Kaci, Yahia ; Benallaoua, Said</creatorcontrib><description>Twenty-three bacterial strains were isolated from root nodules of Spartium junceum L., growing wild in four distinct geographic locations in Bejaia city (Northeastern Algeria). They were analyzed based on their symbiotic effectiveness, phenotypic properties and restriction fragment length polymorphism of the 16S rRNA gene. In addition, sequence analyses of the ribosomal gene, protein-coding housekeeping genes glnII and recA , and symbiotic gene ( nodC ) were used to clarifying their taxonomic and phylogenetic position. Plant infection tests revealed that all strains were able to form nodules on their original host plant, but with variable nodulation ability and symbiotic effectiveness. All strains were slow-growing, alkali-producing rhizobia in YEMA medium. According to phenotypic test results, the strains were grouped in four main clusters in a UPGMA dendrogram, while they were discriminated into 12 distinct genotypes by 16S rDNA-RFLP. Phylogenetic analyses of 16S rRNA genes and two concatenated housekeeping genes, glnII and recA , distributed the representative strains from each RFLP genotypes into two major clades within Bradyrhizobium genus. Some of these strains were closely related to B. retamae and B. japonicum whereas others represented four novel genospecies. Based on analysis of the symbiotic gene sequence ( nodC ), all strains were placed in the symbiovar retamae cluster except for SjBA12 which possibly constitutes a new symbiovar within the genus Bradyrhizobium .</description><identifier>ISSN: 0334-5114</identifier><identifier>EISSN: 1878-7665</identifier><identifier>DOI: 10.1007/s13199-020-00679-8</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Bradyrhizobium ; Developmental Biology ; Ecology ; Evolutionary Biology ; Gene polymorphism ; Genes ; Host plants ; Life Sciences ; Microbiology ; Nodulation ; Phylogenetics ; Phylogeny ; Plant Sciences ; RecA protein ; Restriction fragment length polymorphism ; Root nodules ; rRNA 16S ; Spartium junceum ; Strains (organisms)</subject><ispartof>Symbiosis (Philadelphia, Pa.), 2020-05, Vol.81 (1), p.25-37</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Springer Nature B.V. 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c270t-5409fe708e370e4002eaccdf72cb0c08f9b53a786243997c7e683afaab1025c23</cites><orcidid>0000-0001-5381-4540</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Ramdani, Nacer</creatorcontrib><creatorcontrib>Belhadi, Djellali</creatorcontrib><creatorcontrib>Kaci, Yahia</creatorcontrib><creatorcontrib>Benallaoua, Said</creatorcontrib><title>Symbiotic, phenotypic and genotypic characterization of Bradyrhizobium sp. nodulating Spartium junceum L. from Bejaia, northeastern Algeria</title><title>Symbiosis (Philadelphia, Pa.)</title><addtitle>Symbiosis</addtitle><description>Twenty-three bacterial strains were isolated from root nodules of Spartium junceum L., growing wild in four distinct geographic locations in Bejaia city (Northeastern Algeria). They were analyzed based on their symbiotic effectiveness, phenotypic properties and restriction fragment length polymorphism of the 16S rRNA gene. In addition, sequence analyses of the ribosomal gene, protein-coding housekeeping genes glnII and recA , and symbiotic gene ( nodC ) were used to clarifying their taxonomic and phylogenetic position. Plant infection tests revealed that all strains were able to form nodules on their original host plant, but with variable nodulation ability and symbiotic effectiveness. All strains were slow-growing, alkali-producing rhizobia in YEMA medium. According to phenotypic test results, the strains were grouped in four main clusters in a UPGMA dendrogram, while they were discriminated into 12 distinct genotypes by 16S rDNA-RFLP. Phylogenetic analyses of 16S rRNA genes and two concatenated housekeeping genes, glnII and recA , distributed the representative strains from each RFLP genotypes into two major clades within Bradyrhizobium genus. Some of these strains were closely related to B. retamae and B. japonicum whereas others represented four novel genospecies. Based on analysis of the symbiotic gene sequence ( nodC ), all strains were placed in the symbiovar retamae cluster except for SjBA12 which possibly constitutes a new symbiovar within the genus Bradyrhizobium .</description><subject>Biomedical and Life Sciences</subject><subject>Bradyrhizobium</subject><subject>Developmental Biology</subject><subject>Ecology</subject><subject>Evolutionary Biology</subject><subject>Gene polymorphism</subject><subject>Genes</subject><subject>Host plants</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Nodulation</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Sciences</subject><subject>RecA protein</subject><subject>Restriction fragment length polymorphism</subject><subject>Root nodules</subject><subject>rRNA 16S</subject><subject>Spartium junceum</subject><subject>Strains (organisms)</subject><issn>0334-5114</issn><issn>1878-7665</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kM1OwzAQhC0EEuXnBThZ4krK2k7s5AiIP6kSB-BsbVynddXawU4O5RV4aQxFcOM02tU3s9oh5IzBlAGoy8QEa5oCOBQAUjVFvUcmrFZ1oaSs9skEhCiLirHykByltAIQTAo5IR_P203rwuDMBe2X1odh2ztD0c_p4ncyS4xoBhvdOw4ueBo6eh1xvo1L9x5aN25o6qfUh_m4zoBf0Oce4_C1X43e2KyzKe1i2NBru0KHF5mNw9JiyqGeXq0XORtPyEGH62RPf_SYvN7dvtw8FLOn-8ebq1lhuIKhqEpoOqugtkKBLQG4RWPmneKmBQN117SVQFVLXoqmUUZZWQvsEFsGvDJcHJPzXW4fw9to06BXYYw-n9S8ZA2HSkrIFN9RJoaUou10H90G41Yz0F-l613pOpeuv0vXdTaJnSll2Oev_qL_cX0Ct-yHiA</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Ramdani, Nacer</creator><creator>Belhadi, Djellali</creator><creator>Kaci, Yahia</creator><creator>Benallaoua, Said</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0001-5381-4540</orcidid></search><sort><creationdate>20200501</creationdate><title>Symbiotic, phenotypic and genotypic characterization of Bradyrhizobium sp. nodulating Spartium junceum L. from Bejaia, northeastern Algeria</title><author>Ramdani, Nacer ; Belhadi, Djellali ; Kaci, Yahia ; Benallaoua, Said</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-5409fe708e370e4002eaccdf72cb0c08f9b53a786243997c7e683afaab1025c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biomedical and Life Sciences</topic><topic>Bradyrhizobium</topic><topic>Developmental Biology</topic><topic>Ecology</topic><topic>Evolutionary Biology</topic><topic>Gene polymorphism</topic><topic>Genes</topic><topic>Host plants</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Nodulation</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Sciences</topic><topic>RecA protein</topic><topic>Restriction fragment length polymorphism</topic><topic>Root nodules</topic><topic>rRNA 16S</topic><topic>Spartium junceum</topic><topic>Strains (organisms)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramdani, Nacer</creatorcontrib><creatorcontrib>Belhadi, Djellali</creatorcontrib><creatorcontrib>Kaci, Yahia</creatorcontrib><creatorcontrib>Benallaoua, Said</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Symbiosis (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramdani, Nacer</au><au>Belhadi, Djellali</au><au>Kaci, Yahia</au><au>Benallaoua, Said</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Symbiotic, phenotypic and genotypic characterization of Bradyrhizobium sp. nodulating Spartium junceum L. from Bejaia, northeastern Algeria</atitle><jtitle>Symbiosis (Philadelphia, Pa.)</jtitle><stitle>Symbiosis</stitle><date>2020-05-01</date><risdate>2020</risdate><volume>81</volume><issue>1</issue><spage>25</spage><epage>37</epage><pages>25-37</pages><issn>0334-5114</issn><eissn>1878-7665</eissn><abstract>Twenty-three bacterial strains were isolated from root nodules of Spartium junceum L., growing wild in four distinct geographic locations in Bejaia city (Northeastern Algeria). They were analyzed based on their symbiotic effectiveness, phenotypic properties and restriction fragment length polymorphism of the 16S rRNA gene. In addition, sequence analyses of the ribosomal gene, protein-coding housekeeping genes glnII and recA , and symbiotic gene ( nodC ) were used to clarifying their taxonomic and phylogenetic position. Plant infection tests revealed that all strains were able to form nodules on their original host plant, but with variable nodulation ability and symbiotic effectiveness. All strains were slow-growing, alkali-producing rhizobia in YEMA medium. According to phenotypic test results, the strains were grouped in four main clusters in a UPGMA dendrogram, while they were discriminated into 12 distinct genotypes by 16S rDNA-RFLP. Phylogenetic analyses of 16S rRNA genes and two concatenated housekeeping genes, glnII and recA , distributed the representative strains from each RFLP genotypes into two major clades within Bradyrhizobium genus. Some of these strains were closely related to B. retamae and B. japonicum whereas others represented four novel genospecies. Based on analysis of the symbiotic gene sequence ( nodC ), all strains were placed in the symbiovar retamae cluster except for SjBA12 which possibly constitutes a new symbiovar within the genus Bradyrhizobium .</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s13199-020-00679-8</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-5381-4540</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0334-5114
ispartof Symbiosis (Philadelphia, Pa.), 2020-05, Vol.81 (1), p.25-37
issn 0334-5114
1878-7665
language eng
recordid cdi_proquest_journals_2419205660
source Springer Nature
subjects Biomedical and Life Sciences
Bradyrhizobium
Developmental Biology
Ecology
Evolutionary Biology
Gene polymorphism
Genes
Host plants
Life Sciences
Microbiology
Nodulation
Phylogenetics
Phylogeny
Plant Sciences
RecA protein
Restriction fragment length polymorphism
Root nodules
rRNA 16S
Spartium junceum
Strains (organisms)
title Symbiotic, phenotypic and genotypic characterization of Bradyrhizobium sp. nodulating Spartium junceum L. from Bejaia, northeastern Algeria
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T18%3A02%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Symbiotic,%20phenotypic%20and%20genotypic%20characterization%20of%20Bradyrhizobium%20sp.%20nodulating%20Spartium%20junceum%20L.%20from%20Bejaia,%20northeastern%20Algeria&rft.jtitle=Symbiosis%20(Philadelphia,%20Pa.)&rft.au=Ramdani,%20Nacer&rft.date=2020-05-01&rft.volume=81&rft.issue=1&rft.spage=25&rft.epage=37&rft.pages=25-37&rft.issn=0334-5114&rft.eissn=1878-7665&rft_id=info:doi/10.1007/s13199-020-00679-8&rft_dat=%3Cproquest_cross%3E2419205660%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c270t-5409fe708e370e4002eaccdf72cb0c08f9b53a786243997c7e683afaab1025c23%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2419205660&rft_id=info:pmid/&rfr_iscdi=true