Cys-functionalized AuNP substrates for improved sensing of the marine toxin STX by dynamic surface-enhanced Raman spectroscopy

Saxitoxin (STX) as one of the most harmful and typical paralytic shellfish toxins, is posing a serious threat to environmental and human health, thus it is essential to develop a sensitive and reliable analytical method for STX detection. Herein, we proposed a strategy for rapid and sensitive detect...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2020-07, Vol.412 (19), p.4609-4617
Main Authors: Cao, Chentai, Li, Pan, Liao, Hongmei, Wang, Junping, Tang, Xianghu, Yang, Liangbao
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Biosensing Techniques - methods
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Cysteine
Cysteine - chemistry
Dynamic stability
Electrostatic properties
Food Science
Gold
Gold - chemistry
Hydrogen
Hydrogen bonding
Hydrogen bonds
Laboratory Medicine
Limit of Detection
Marine toxins
Metal Nanoparticles - chemistry
Monitoring/Environmental Analysis
Nanoparticles
Povidone
Raman spectroscopy
Research Paper
Saxitoxin
Saxitoxin - analysis
Sensitivity
Shellfish
Spectroscopy
Spectrum analysis
Spectrum Analysis, Raman - methods
Strategy
Substrates
Surface stability
Toxins
Water Pollutants, Chemical - analysis
Zeta potential
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Summary:Saxitoxin (STX) as one of the most harmful and typical paralytic shellfish toxins, is posing a serious threat to environmental and human health, thus it is essential to develop a sensitive and reliable analytical method for STX detection. Herein, we proposed a strategy for rapid and sensitive detection of STX with surface-enhanced Raman spectroscopy (SERS), by employing cysteine modified gold nanoparticles (Cys-AuNPs) as SERS probe to capture STX molecules through electrostatic interactions and multiple hydrogen bonds between Cys and STX molecules. Moreover, the XPS and zeta potential results indicated that Cys could bond to AuNPs through Au-S bonds and the addition of STX could induce the efficient aggregation of Cys-AuNPs owing to the presence of electrostatic interactions and multiple hydrogen bonds between Cys and STX molecules. Furthermore, considering the high sensitivity and stability of the dynamic surface-enhanced Raman spectroscopy (D-SERS) strategy with the formation of a 3D hotspot matrix, the highly sensitive detection of STX was realized to a level of 1 × 10 −7  M by using the D-SERS strategy. Consequently, Cys-AuNPs as high affinity substrates can provide high sensitivity for the detection of STX through the D-SERS strategy. Graphical abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-020-02710-9