Growth Kinetics and Spoilage Potential of Co-culturing Acinetobacter johnsonii and Pseudomonas fluorescens from Bigeye Tuna (Thunnus obesus) During Refrigerated Storage

A. johnsonii and P. fluorescens are the well-known specific spoilage organisms in aquatic products and the study of the interactions between A. johnsonii and P. fluorescens are limited. This study aims to evaluate the growth kinetics, spoilage potential and interactions of  A. johnsonii and  P. fluo...

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Bibliographic Details
Published in:Current microbiology 2020-08, Vol.77 (8), p.1637-1646
Main Authors: Wang, Xin-Yun, Xie, Jing
Format: Article
Language:English
Subjects:
Acinetobacter - growth & development
Animals
Atomic force microscopy
Atomic properties
Biodegradation
Biomedical and Life Sciences
Biotechnology
Cold storage
Correlation coefficient
Correlation coefficients
Fish
Food Microbiology
Food Storage
Growth kinetics
Inoculation
Kinetics
Life Sciences
Microbial Interactions
Microbiology
Microorganisms
Mixed culture
Nitrogen
pH effects
Proteins
Proteolysis
Pseudomonas fluorescens
Pseudomonas fluorescens - growth & development
Raw Foods - microbiology
Refrigeration
Seafood - microbiology
Spoilage
Structural damage
Thunnus obesus
Trimethylamine
Tuna
Tuna - microbiology
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Summary:A. johnsonii and P. fluorescens are the well-known specific spoilage organisms in aquatic products and the study of the interactions between A. johnsonii and P. fluorescens are limited. This study aims to evaluate the growth kinetics, spoilage potential and interactions of  A. johnsonii and  P. fluorescens isolated from spoiled bigeye tuna ( Thunnus obesus ) by inoculating into sterile fish slices and stored at 4 °C for 6 days. The growth kinetics of A. johnsonii and P. fluorescens were fitted with Baranyi and Roberts model. The chemical indexes (total volatile base nitrogen (TVB-N), trimethylamine (TMA), pH, proteolytic activity and protein content) of each inoculated block of bigeye tuna were increased during refrigerated storage. Moreover, the higher contents of chemical indexes were observed in co-culture with A. johnsonii and P. fluorescens compared with single culture of A. johnsonii and P. fluorescens . In addition, atomic force microscopy (AFM) observation of co-culturing A. johnsonii and P. fluorescens inoculation into sterile fish slices revealed damage of myofibrillar protein structures and the protein degradation. Based on these parameters, a rapid method to evaluate spoilage potential of A. johnsonii and P. fluorescens was positively correlated with TVB-N value, TMA value and pH value ( P  
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-020-01978-5