A novel dicationic Quinoline-Carzole fluorescent probe: preparation and labelling of Microthrix parvicella

A novel dicationic Quinoline-Carbazole fluorescent probe with hydrophobic long-chain alkane was designed and synthesized based on the property of Microthrix parvicella (M. parvicella) in situ utilizing long-chain fatty acids (LCFA) in the activated sludge system. 1 H NMR spectrum, ultraviolet-visibl...

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Bibliographic Details
Published in:Environmental technology 2020-08, Vol.41 (18), p.2393-2399
Main Authors: Fei, Xuening, Xing, Yanjun, Zhang, Buqing, Zhu, Sen, Liu, Lijuan
Format: Article
Language:English
Subjects:
Absorption spectra
Activated sludge
Alkanes
Candidatus Microthrix parvicella
Carbazole
Carbazoles
Characterization
Dicationic Quinoline-Carbazole
Fatty acids
Fish oils
Fluorescence
Fluorescent indicators
fluorescent probe
Hydrophobicity
Labeling
labelling
Microthrix parvicella
NMR
Nuclear magnetic resonance
Quinoline
Ultraviolet spectra
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Summary:A novel dicationic Quinoline-Carbazole fluorescent probe with hydrophobic long-chain alkane was designed and synthesized based on the property of Microthrix parvicella (M. parvicella) in situ utilizing long-chain fatty acids (LCFA) in the activated sludge system. 1 H NMR spectrum, ultraviolet-visible (UV-Vis) absorption spectra, and fluorescent spectra analysis demonstrated that the probe was successfully obtained. The probe had a large stokes-shift ranging from 102 to 144 nm in different solvents, which were benefit for the fluorescent labelling properties. The labelling experiment indicated that the prepared probe could absorb onto the surface of M. parvicella through hydrophobic bond. Much stronger yellow fluorescence of M. parvicella was observed at the concentration of 1.0 × 10 −5  mol/L when compared with the zooglea, which makes it easy to distinguish M. parvicella from the zooglea. In addition, the photostability of the probe was also investigated, and the result showed that the probe was quite stable in a long period of time. All the results indicated that the prepared probe was suitable for the labelling of M. parvicella.
ISSN:0959-3330
1479-487X
DOI:10.1080/09593330.2019.1567606