An Investigation of Efficient Detection of Ultra-Low Concentration of Penicillins in Milk Using AuNPs/PSi Hybrid Structure

• Published in: Plasmonics (Norwell, Mass.) Mass.), 2020-08, Vol.15 (4), p.985-993
• Main Authors: Wali, Layla A., Hasan, Khulood K., Alwan, Alwan M.
• Format: Article
• Language: English
• Subjects: Ampicillin; Biochemistry; Biological and Medical Physics; Biophysics; Biotechnology; Chemistry; Chemistry and Materials Science; Correlation coefficients; Gold; Hybrid structures; Milk; Nanoparticles; Nanotechnology; Penicillin; Porous silicon; Residues
• ISSN: 1557-1955; 1557-1963
• DOI: 10.1007/s11468-019-01096-4

In this work, porous silicon (PSi) sample was employed to increase the SERS efficiency for rapid detection of penicillins in spiked milk by developing Au nanoparticles (AuNPs)/PSi hybrid structure. SERS was used to study penicillin G and ampicillin residue in milk. The results show that the AuNPs/PSi hybrid structure is very able to detect penicillins residue in milk with an excellent linear relationship and a correlation coefficient ( R 2  = 1) in the range of 1 × 10 −7  mol/L to 1 × 10 −9  mol/L. The highest enhancement factors of penicillin G (2.8 × 10 8 ) and of ampicillin (1.2 × 10 8 ) with an excellent relative standard deviation (RSD) of 2.69 and 0.93, respectively, are obtained at the ultra-low concentration of 1 × 10 −9  mol/L. The detection limit of penicillin G and ampicillin is 1 × 10 −9  mol/L (equal to 0.33 μg/kg and 0.35 μg/kg, respectively) which is very lower than the maximum residue limit (MRL) of penicillins in milk (4 μg/kg) established by the European Union.