The impact of fruit fly gut bacteria on the rearing of the parasitic wasp Diachasmimorpha longicaudata

• Published in: Entomologia experimentalis et applicata 2020-07, Vol.168 (6-7), p.541-559
• Main Authors: Koskinioti, Panagiota, Ras, Erica, Augustinos, Antonios A., Beukeboom, Leo W., Mathiopoulos, Kostas D., Caceres, Carlos, Bourtzis, Kostas
• Format: Article
• Language: English
• Subjects: Agricultural practices; area‐wide integrated pest management; Bacteria; Biological control; Braconidae; Diachasmimorpha longicaudata; Enterobacter; Fecundity; Females; Fitness; Fruit flies; Fruits; Hymenoptera; Integrated pest management; Klebsiella; Larvae; Mass rearing; Mediterranean fruit fly; microbiome; Offspring; olive fruit fly; parasitic wasp; Parasitism; parasitoid; Parasitoids; Pest control; Progeny; SIT; sterile insect technique; Symbionts; symbiosis; Tephritidae
• ISSN: 0013-8703; 1570-7458
• DOI: 10.1111/eea.12936

Area‐wide integrated pest management strategies against tephritid fruit flies include the release of fruit fly parasitic wasps in the target area. Mass rearing of parasitic wasps is essential for the efficient application of biological control strategies. Enhancement of fruit fly host fitness through manipulation of their gut‐associated symbionts might also enhance the fitness of the produced parasitic wasps and improve the parasitoid rearing system. In the current study, we added three gut bacterial isolates originating from Ceratitis capitata (Wiedemann) and four originating from Bactrocera oleae (Rossi) (both Diptera: Tephritidae) to the larval diet of C. capitata and used the bacteria‐fed larvae as hosts for the development of the parasitic wasp Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae). We evaluated the effect of the bacteria on wasp life‐history traits and assessed their potential use for the improvement of D. longicaudata rearing. Enterobacter sp. AA26 increased fecundity and parasitism rate and accelerated parasitoid emergence. Providencia sp. AA31 led to faster emergence of both male and female parasitoids, whereas Providencia sp. 22 increased the production of female progeny. Bacillus sp. 139 increased parasitoid fecundity, parasitism rate, and production of female progeny. Serratia sp. 49 accelerated parasitoid emergence for both males and females and increased production of female progeny. Klebsiella oxytoca delayed parasitoid emergence and Enterobacter sp. 23 decreased parasitoid fecundity and parasitism rate. Our findings demonstrate a wide range of effects of fruit fly gut symbionts on parasitoid production and reveal a great potential of bacteria use towards enhancement of parasitic wasp rearing. We assessed the potential use of three gut bacterial isolates originating from Mediterranean fruit fly (medfly), Ceratitis capitata, and four originating from olive fruit fly, Bactrocera oleae (both Diptera: Tephritidae), as supplements to the larval diet of C. capitata for improving the rearing of Diachasmimorpha longicaudata (Hymenoptera: Braconidae). Our findings demonstrate a wide range of effects of fruit fly gut symbionts on parasitoid production and reveal a great potential of bacteria use towards enhancement of parasitic wasp rearing.