Persistence of diet effects on the microbiota of Drosophila suzukii (Diptera: Drosophilidae)

The insect commensal microbiota consists of prokaryotes and eukaryotes. We explored the effect of diet and the persistence of the gut microbiota across generations in Drosophila suzukii (Matsumura) (Diptera: Drosophilidae). We transferred subsets of a single population of D. suzukii to different fru...

Full description

Saved in:
Bibliographic Details
Published in:Canadian entomologist 2020-08, Vol.152 (4), p.516-531
Main Authors: Jiménez-Padilla, Yanira, Esan, Ebenezer O., Floate, Kevin D., Sinclair, Brent J.
Format: Article
Language:English
Subjects:
Bacteria
Blueberries
Data analysis
Deoxyribonucleic acid
Diet
Diptera
DNA
Drosophila suzukii
Drosophilidae
Eggs
Entomology
Eukaryotes
Females
Fragaria ananassa
Fruits
Fungi
Genomes
Genomics
Insects
Intestinal flora
Intestinal microflora
Laboratories
Microbiota
Physiology
Population
Prokaryotes
Research Papers
Rosaceae
rRNA 16S
Rubus
Species diversity
Strawberries
Vaccinium
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The insect commensal microbiota consists of prokaryotes and eukaryotes. We explored the effect of diet and the persistence of the gut microbiota across generations in Drosophila suzukii (Matsumura) (Diptera: Drosophilidae). We transferred subsets of a single population of D. suzukii to different fruit-based diets (blueberry (Vaccinium Linnaeus; Ericaceae), raspberry (Rubus Linnaeus; Rosaceae), and strawberry (Fragaria × ananassa Duchesne; Rosaceae)) for three generations and then returned them to a common, banana-based, diet. We used 16S rDNA (Bacteria) and ITS (internal transcribed spacer; Fungi) sequencing of female endosymbiont-free flies to identify the microbiota. We identified 2700 bacterial and 350 fungal operational taxonomic units (OTUs); there was no correlation between the number of bacterial and fungal OTUs in a sample. Bacterial communities were dominated by Proteobacteria (especially Acetobacteraceae); Ascomycota dominated the fungal communities. Species diversity of both bacteria and fungi differed among diets, but there were no differences in species-level diversity when these D. suzukii were returned to a control diet. A principle coordinates analysis revealed no differences in the bacterial or fungal community in the first generation on fruit diets, but that the communities diverged over the next two generations; neither fungal and bacterial communities converged after one generation on control food. We conclude that diet changes the D. suzukii microbiota, and that these changes persist for more than one generation.
ISSN:0008-347X
1918-3240
DOI:10.4039/tce.2020.37