Biological parameters for Rhipicephalus microplus in the field and laboratory and estimation of its annual number of generations in a tropical region

• Published in: Parasitology research (1987) 2020-08, Vol.119 (8), p.2421-2430
• Main Authors: Cruz, Breno Cayeiro, de Lima Mendes, Ana Flávia, Maciel, Willian Giquelin, dos Santos, Isabella Barbosa, Gomes, Lucas Vinicius Costa, Felippelli, Gustavo, Teixeira, Weslen Fabricio Pires, Ferreira, Lorena Lopes, Soares, Vando Edésio, Lopes, Welber Daniel Zanetti, da Costa, Alvimar José, de Oliveira, Gilson Pereira
• Format: Article
• Language: English
• Subjects: Animals; Arthropods and Medical Entomology - Original Paper; Biomedical and Life Sciences; Biomedicine; Cattle; Climate; Climatic conditions; Female; Hatching; Immunology; Laboratories; Larva; Life Cycle Stages - physiology; Longevity; Medical Microbiology; Microbiology; Oviposition; Pasture; Rainfall; Rhipicephalus - physiology; Rhipicephalus microplus; Soil temperature; Time Factors; Tropical environment
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-020-06758-5

The present study aimed to evaluate biological parameters of Rhipicephalus microplus in the non-parasitic phase in both field and laboratory conditions. It also aimed to assess correlations between duration (in days) of these parameters and climatic variables (humidity, rainfall, and soil temperature) and to estimate the annual number of generations of R. microplus in a tropical region. The non-parasitic phase of R. microplus in field and laboratory conditions was evaluated throughout the course of two years. A pasture was infested with engorged female of R. microplus , and biological parameters, including female pre-oviposition, female oviposition, egg mass incubation, larval pre-hatching phase, larval maturation, and larval longevity, were evaluated concomitantly with the collection of data on climatic conditions. The same parameters were also evaluated in a climatized chamber in the laboratory. The total duration of the non-parasitic phase in the field was longer in the dry season (1st and 4th life-cycle repetitions) than in the rainy season (2nd, 3rd, and 5th repetitions). Tick biological parameters for the non-parasitic phase in the laboratory were similar to those obtained in the field during the rainy season. The evaluated biological parameters were influenced mainly by environmental and ground-level temperatures, which modified egg mass incubation, larval pre-hatching, and larval longevity periods and, consequently, the total duration of the non-parasitic phase of the tick. The annual number of generations for the tick was estimated at five per year, which is alarming because it represents an increase, and so new studies into strategic control are needed.