No antibiotic and toxic metabolites produced by the biocontrol agent Pseudomonas putida strain B2017

ABSTRACT Pseudomonas putida and closely-related species such as Pseudomonas fluorescens and Pseudomonas brassicacearum have been reported as potential biocontrol agents and plant growth-promoters. Recently, we have described the biocontrol activity of P. putida B2017 against several phytopathogens o...

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Bibliographic Details
Published in:FEMS microbiology letters 2020-05, Vol.367 (9), p.1
Main Authors: Daura-Pich, Oriol, Hernández, Iker, Pinyol-Escala, Lola, Lara, Jose M, Martínez-Servat, Sonia, Fernández, Carolina, López-García, Belén
Format: Article
Language:English
Subjects:
Antibiotics
Biological control
Biosynthesis
Cyanides
Environmental effects
Genomic analysis
Lipopeptides
Mass spectrometry
Mass spectroscopy
Metabolites
Microbiological research
Microbiology
Mode of action
Physiological aspects
Plant growth
Plant protection
Pseudomonas fluorescens
Pseudomonas putida
Pyocyanin
Pyoverdines
Side effects
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Summary:ABSTRACT Pseudomonas putida and closely-related species such as Pseudomonas fluorescens and Pseudomonas brassicacearum have been reported as potential biocontrol agents and plant growth-promoters. Recently, we have described the biocontrol activity of P. putida B2017 against several phytopathogens of agricultural relevance. In this study, its ability to produce potential antibiotic / toxic metabolites was assessed by functional, chromatography-mass spectrometry and genomic analysis. Our results show that B2017 is not able to synthesize surfactants and common antibiotics produced by Pseudomonas spp., i.e. pyrrolnitrin, 2,4-diacetylphloroglucinol, pyoluteorin and pyocyanin, but it produces pyoverdine, a siderophore which is involved in its biocontrol activity. The non-production of other metabolites, such as cyanide, safracin, promysalin and lipopeptides between others, is also discussed. Our data suggest that the mode of action of B2017 is not mainly due to the production of antimicrobial / toxic metabolites. Moreover, these features make P. putida B2017 a promising biocontrol microorganism for plant protection without side effects on environment, non-target organisms and human health. The non-production of antibiotics / toxic metabolites by Pseudomonas putida strain B2017 makes it a promising biocontrol microorganism for plant protection without side effects on environment, non-target organisms, and human health.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnaa075