Protective Antioxidant Effects of Amentoflavone and Total Flavonoids from Hedyotis diffusa on H2O2‐Induced HL‐O2 Cells through ASK1/p38 MAPK Pathway

In this study, total flavonoids and total triterpenoid acid were extracted with ethyl acetate from Hedyotis diffusa Willd, and hepatoprotective activities of them and five compounds from total flavonoids against H2O2 induced hepatocyte damage on HL‐02 cells were determined. In particular, amentoflav...

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Published in:Chemistry & biodiversity 2020-07, Vol.17 (7), p.n/a
Main Authors: Li, Yun‐Lan, Chen, Xi, Niu, Si‐Qing, Zhou, Hong‐Yan, Li, Qing‐Shan
Format: Article
Language:English
Subjects:
Acetic acid
Antioxidants
Caspase
Ethyl acetate
Flavonoids
Hedyotis
Hedyotis diffusa
Hepatotoxicity
Hydrogen peroxide
MAP kinase
Oxidative stress
Reductase
Reductases
Streaming
Thioredoxin
Trx, ASK1/P38 MAPK signaling pathway
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Summary:In this study, total flavonoids and total triterpenoid acid were extracted with ethyl acetate from Hedyotis diffusa Willd, and hepatoprotective activities of them and five compounds from total flavonoids against H2O2 induced hepatocyte damage on HL‐02 cells were determined. In particular, amentoflavone and total flavonoids had influence on the leakage of ALT, AST, LDH, the activities of SOD and the content of MDA. They effectively reduced the loss of MMP, the release of Cyt C, and then inhibited activation of caspase‐3/caspase‐9 cascade in hepatotoxic cells. The contents of ROS were significantly reduced to inhibit p38 in amentoflavone and flavonoids groups which decreased ASK1 and p‐p38 levels through increasing thioredoxin Trx1 and reductase TrxR1. These results suggesting that the antioxidant protection of amentoflavone and flavonoids might be reducing ROS to inhibit the H2O2‐induced upstream of pathway via increasing levels of Trx1 and TrxR1, which were pivotal in blocking the down streaming effectors of ASK1/p38 MAPK pathway and alleviating hepatotoxicity.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202000251