Fluorescence of chlorophyll a in transgenic maize with herbicide application and attacked by Spodoptera frugiperda (Lepidoptera: Noctuidae)

• Published in: Phytoparasitica 2020-09, Vol.48 (4), p.567-573
• Main Authors: de Souza, Michael Willian Rocha, Ferreira, Evander Alves, dos Santos, José Barbosa, Soares, Marcus Alvarenga, de Castro e Castro, Bárbara Monteiro, Zanuncio, José Cola
• Format: Article
• Language: English
• Subjects: Agriculture; Atrazine; Biomedical and Life Sciences; Chlorophyll; Corn; Damage; Ecology; Electron transport; Fluorescence; Genotypes; Herbicides; Life Sciences; Pests; Physiology; Plant Pathology; Plant Sciences; Proteins; Spodoptera frugiperda; Stress (physiology); Transport rate
• ISSN: 0334-2123; 1876-7184
• DOI: 10.1007/s12600-020-00816-5

Physiological changes in maize plants, submitted to herbicides and pests, should be studied for integrated management in this crop. The objective was to evaluate the chlorophyll a fluorescence in maize genotypes damaged or not by Spodoptera frugiperda , and sprayed with herbicides. The herbicides atrazine and nicosulfuron were applied to the plants at doses of 3 kg ha − 1 and 0.06 kg ha − 1 , respectively. A randomized block design in a factorial scheme with an additional treatment 3 (2 × 2 + 1) was used, being the factor A: maize leaves exposed or not to the attack of S. frugiperda ; factor B: Herculex® (transgenic maize encoding the Cry1F protein) and Isohybrid (non-transgenic maize) genotypes and an additional treatment represented by the PowerCore® genotype (stacked transgenic maize encoding the Cry1F, Cry1A.105 and Cry2Ab2 proteins). The initial fluorescence of chlorophyll a (F0 - electrons quantum − 1 ), maximum fluorescence (Fm - electron quantum − 1 ), ratio between variable fluorescence and maximum fluorescence of chlorophyll a (Fv/Fm) and electron transport rate ETR-µmols electrons m − 2 s − 1 ) were analyzed. The initial fluorescence of chlorophyll a increased and Fm, Fv/Fm and ETR decreased in plants attacked by the pest. The PowerCore® chlorophyll fluorescence remained in characteristic patterns. Chlorophyll a fluorescence was similar between genotypes with or without herbicides application. Damage by S. frugiperda increased the initial fluorescence of chlorophyll a and reduced the variable fluorescence/maximum fluorescence ratio and electron transport rate, indicating physiological stress in maize plants. The herbicides do not cause physiological stress in plants of maize genotypes with characteristic values of chlorophyll a fluorescence.