Incorporation of Mannitol in Tissue Culture Media for Long-Term Storage of Potatoes at Moderately Low Temperature and Effect on Subsequent Micropropagation

Long-term maintenance in vitro of potato plantlets in nutrient media is necessary to preserve disease-free stocks. Since these are living plants, they eventually outgrow the containers they are in and exhaust the media, so propagation onto fresh media is required. To decrease labor and time spent in...

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Bibliographic Details
Published in:American journal of potato research 2020-08, Vol.97 (4), p.439-446
Main Authors: Chappell, Angel L., Koym, Jeffrey W., Scheuring, Douglas C., Miller, J. Creighton, Vales, M. Isabel
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Containers
Culture media
Genotypes
Life Sciences
Low temperature
Mannitol
Micropropagation
Osmotic stress
Plant breeding
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant growth
Plant Pathology
Plant Sciences
Plantlets
Potatoes
Propagation
Regeneration
Short Communication
Tissue culture
Vegetables
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Summary:Long-term maintenance in vitro of potato plantlets in nutrient media is necessary to preserve disease-free stocks. Since these are living plants, they eventually outgrow the containers they are in and exhaust the media, so propagation onto fresh media is required. To decrease labor and time spent in this process, it is of interest to slow plant growth to reduce turnover frequency. In this study, we investigated the effects of adding two concentrations (2 and 4%) of mannitol (a sugar alcohol that causes osmotic stress) to tissue culture media on plant growth of three potato clones at moderately low temperatures. Plantlet growth was inversely proportional to the concentration of mannitol used, but there were genotypic differences. Regeneration of the plantlets for subsequent micropropagation, after removal from the main treatments at day 64 and 121 days, was not negatively affected by the percentage of mannitol used to maintain the original stocks. Based on these results, we started using 2% mannitol as a baseline for practical long-term storage of potato breeding clones maintained in vitro. Depending on the reaction of the specific genotypes to media containing mannitol, additional adjustments might be needed.
ISSN:1099-209X
1874-9380
DOI:10.1007/s12230-020-09780-6