Isolation, characterisation and cross amplification of thirteen microsatellite loci for coral endo-symbiotic dinoflagellates (Symbiodinium clade C)

The health and productivity of coral reefs is underpinned by the symbiosis between corals and dinoflagellates ( Symbiodinium spp.). To enable population genetic analyses of Symbiodinium spp, required for coral reef conservation, seven microsatellite loci were isolated from Symbiodinium clade C from...

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Bibliographic Details
Published in:Conservation genetics resources 2009-12, Vol.1 (1), p.199-203
Main Authors: Bay, Line Kolind, Howells, Emily J., van Oppen, Madeleine J. H.
Format: Article
Language:English
Subjects:
Alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Coral reefs
Corals
Dinoflagellata
Dinoflagellates
Ecology
Evolutionary Biology
Genetic analysis
Genetic diversity
Genetic structure
Life Sciences
Microorganisms
Plant Genetics and Genomics
Population genetics
Symbiodinium
Symbiosis
Technical Note
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Summary:The health and productivity of coral reefs is underpinned by the symbiosis between corals and dinoflagellates ( Symbiodinium spp.). To enable population genetic analyses of Symbiodinium spp, required for coral reef conservation, seven microsatellite loci were isolated from Symbiodinium clade C from the Great Barrier Reef, Australia. These microsatellite primer pairs consistently amplified between 1 and 8 alleles per coral host colony, with mean number of alleles ranging from 1.9 to 4.0 and generally high genetic diversities (Shannon’s Index = 0.71–2.76). The novel microsatellite loci amplified between 1 and 10 alleles in four other C strains, but did not amplify a D strain . Three of six previously published clade C microsatellite loci amplified 1–6 alleles in two or more of five C strains tested. The primers and cross amplifications presented here therefore provide a useful tool for elucidating the population genetic structure of clade C Symbiodinium populations.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-009-9048-1