Fumonisin B1 Affects the Biophysical Properties, Migration and Cytoskeletal Structure of Human Umbilical Vein Endothelial Cells

• Published in: Cell biochemistry and biophysics 2020-09, Vol.78 (3), p.375-382
• Main Authors: Zhao, Xue, Wang, Yun, Liu, Jiang-Li, Zhang, Jian-Hua, Zhang, Shi-Chao, Ouyang, Yan, Huang, Jiang-Tao, Peng, Xiao-Yan, Zeng, Zhu, Hu, Zu-Quan
• Format: Article
• Language: English
• Subjects: Actin; Actins - metabolism; Animal health; Binding; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biotechnology; Carcinogenicity; Carcinogens; Cell Biology; Cell membranes; Cell migration; Cell Movement - drug effects; Cell surface; Cell Survival; Cell viability; Cytoskeleton; Cytoskeleton - drug effects; Endothelial cells; Enzyme Inhibitors - pharmacology; Fluidity; Fumonisin B1; Fumonisins - pharmacology; Fusarium; Gene expression; Human Umbilical Vein Endothelial Cells - drug effects; Humans; Immunofluorescence; Life Sciences; Membrane fluidity; Molecular modelling; Mycotoxins; Original Paper; Osmotic Fragility; Pharmacology/Toxicology; Proteins; Reactive oxygen species; Reactive Oxygen Species - metabolism; Real-Time Polymerase Chain Reaction; Toxicity; Umbilical vein
• ISSN: 1085-9195; 1559-0283
• DOI: 10.1007/s12013-020-00923-4

Fumonisin B1 (FB1) is an important mycotoxin in nature and is a serious threat to human and animal health, but its specific target and molecular mechanism of the toxicity and potential carcinogenicity remain unclear. In this study, we first detected the effects of FB1 on the cell viability, biophysical properties, migration ability, and reactive oxygen species (ROS) of human umbilical vein endothelial cells (HUVECs). Subsequently, changes in the cytoskeletal structure and its binding proteins were analyzed by immunofluorescence and real-time PCR, respectively. The results showed that FB1 could inhibit the viability of HUVECs in a dose-dependent manner. After treatment of HUVECs with FB1, the hypotonic resistance, cell surface charges, cell membrane fluidity, and migration ability were weakened, whereas the ROS levels were significantly increased. Moreover, the cytoskeletal structure of the HUVECs was significantly changed, and the mRNA expression of some important actin-binding proteins was altered. Therefore, this study revealed that FB1 can affect the migration and cytoskeletal structure of HUVECs, which provides a new perspective for further understanding the molecular mechanisms of FB1 toxicity.