Meiotic block with roscovitine improves competence of porcine oocytes by fine‐tuning activities of different cyclin‐dependent kinases

Successful use of oocytes from small follicles (SFs) is of great importance for animal embryo production and human in vitro fertilization with reduced hormone‐related side effects. How in vitro meiotic arrest maintenance (MAM) increases the competence of oocytes is not clear. In this study, pig oocy...

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Bibliographic Details
Published in:Journal of cellular physiology 2020-10, Vol.235 (10), p.7530-7540
Main Authors: Zhu, Shuai, Jia, Ying‐Jun, Pan, Liu‐Zhu, Gong, Shuai, Sun, Ming‐Ju, Wang, Guo‐Liang, Luo, Ming‐Jiu, Tan, Jing‐He
Format: Article
Language:English
Subjects:
Abattoirs
Animals
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Chromatin
Chromatin - metabolism
Condensates
Cyclic AMP
Cyclin-dependent kinase 2
Cyclin-dependent kinase 5
Cyclin-Dependent Kinases - metabolism
cyclin‐dependent kinase
Embryo, Mammalian - drug effects
Embryo, Mammalian - metabolism
Embryonic Development - drug effects
Embryos
Female
Follicles
Gametocytes
In vitro fertilization
In Vitro Oocyte Maturation Techniques - methods
Kinases
Meiosis
Meiosis - drug effects
Nucleoli
oocyte competence
Oocytes
Oocytes - drug effects
Oocytes - metabolism
Ovaries
pig
Roscovitine
Roscovitine - pharmacology
Side effects
Swine
Transcription
Transcription, Genetic - drug effects
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Summary:Successful use of oocytes from small follicles (SFs) is of great importance for animal embryo production and human in vitro fertilization with reduced hormone‐related side effects. How in vitro meiotic arrest maintenance (MAM) increases the competence of oocytes is not clear. In this study, pig oocytes recovered from SF of 1–2 mm and medium‐follicles (MF) of 3–6 mm in diameter from abattoir ovaries were treated by various MAM treatments to improve their competence. The results showed that 25 µM roscovitine or 1 mM db‐cAMP efficiently blocked germinal vesicle breakdown in both SF and MF oocytes suggesting a similar cyclin‐dependent kinase (CDK) 1 level between the two oocyte groups. MAM with 15‐ and 25‐µM roscovitine alone or with 1‐mM db‐cAMP improved competence of SF and MF oocytes, respectively, with a promoted chromatin configuration transition from surrounded nucleoli (SN) to re‐decondensation (RDC) pattern that supported substantial gene transcription. However, MAM with db‐cAMP alone or with higher concentrations of roscovitine did not improve oocyte competence, could not support an SN‐to‐RDC transition, and/or evoked a premature chromatin condensation (PMC) that suppressed gene transcription. Both CDK2 and CDK5 contents were higher (p 
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.29655