Potential activity of Madhuca longifolia leaf extract: through In vitro , Pharmacological and In silico studies

The ligands like myricetin 3-O-L-rhamnoside, quercetin 3-galactoside, a-D-Mannopyranoside, D-Allose, myricetin, Quercetin, myricetin 3- O-arabinoside and 3'-Hydroxy-4'-methoxydiclofenac has M.longifolia potential binding affinity with the receptors. [...]the antioxidant, pharmacological an...

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2020-03, Vol.13 (3), p.1083-1091
Main Authors: Peter, S Jerine, Kumar, K Ram, Manisha, P, Sangeetha, N, Raj, N Arun, Kumari, Usha, Sabina, Evan Prince
Format: Article
Language:English
Subjects:
Acids
Analgesics
Antioxidants
Drug dosages
Free radicals
Leaves
Ligands
Metabolites
Methods
Rheumatism
Software
Statistical analysis
Toxicology
Variance analysis
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Summary:The ligands like myricetin 3-O-L-rhamnoside, quercetin 3-galactoside, a-D-Mannopyranoside, D-Allose, myricetin, Quercetin, myricetin 3- O-arabinoside and 3'-Hydroxy-4'-methoxydiclofenac has M.longifolia potential binding affinity with the receptors. [...]the antioxidant, pharmacological and potential binding affinity of M.longifolia leaf was demonstrated in our research. Natural enzymes like superoxide dismutase, Catalase, reduced glutathione, glutathione peroxidase are present in the body which degrades the accumulation of free radical that prevent the system from the formation of oxidative stress7. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) is commonly used to estimate the antioxidant activity of the plant extract. Group-I is normal control, group-II is treated with indomethacin (10mg/kg/b.wt., i.p), group-III is treated with aqueous leaf extract of Madhuca longifolia (ALMEL) at the dosage of 500mg/kg/b.wt., p.o., groupIV is treated with ethanolic leaf extract of Madhuca longifolia (ELMEL) at the dosage of 500mg/kg/b.wt., p.o. and group-V is treated with methanolic leaf extract of Madhuca longifolia (MLEML) at the dosage of 500 mg/kg/b.wt., p.o. The pharmacological activity was analysed through Hot- Plate test, Analgesic Test16, Antipyretic Test17, Ulcerogenic Test18, Stomach and intestine Mucosa. 2.3 Computational analysis: 2.3.1 Receptor designing: The PDB Id for each receptor is Apo Human Pregnane X Receptor (PXR): 1IlG, Constitutive androstane receptor (CAR): 1XNX, Nuclear bile acid receptor (FXR): 1OSH, Liver X receptors alpha (LXR): 5AVI and Nuclear factor kappalight-chain-enhancer of activated B cells (Nf-kB): 1NFK.
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.5958/0974-360X.2020.00199.7