A Validated RP-HPLC Method for the Simultaneous Estimation of Atorvastatin calcium and Clopidogrel Bisulphate in Combined Dosage Forms

The mobile phase flow rate was 1ml/min and the eluents were detected at 245nm.Using the optimized chromatographic conditions, resolved sharp peaks corresponding to Atorvastatin Calcium and Clopidogrel Bisulphate could be obtained at retention time of 2.007 and 5.977min respectively. [...]the aim of...

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2020-03, Vol.13 (3), p.1227-1230
Main Authors: Jose, Elseena, Shyamkumar, B, Jose, Manu
Format: Article
Language:English
Subjects:
Accuracy
Acids
Chromatography
Drug dosages
Pharmaceuticals
Sensors
Standard deviation
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Summary:The mobile phase flow rate was 1ml/min and the eluents were detected at 245nm.Using the optimized chromatographic conditions, resolved sharp peaks corresponding to Atorvastatin Calcium and Clopidogrel Bisulphate could be obtained at retention time of 2.007 and 5.977min respectively. [...]the aim of the study was to develop a rapid, sensitive, simple and precise RP-HPLC method which can estimate both the drugs simultaneously. In this work an isocratic, simple, accurate and sensitive RP-HPLC method suitable for the simultaneous determination of Atorvastatin Calcium and Clopidogrel Bisulphate in capsule dosage form has been developed.Initially various mobile phases with different ratios were investigated in an attempt to obtain best resolution for Atorvastatin calcium and Clopidogrel Bisulphate. Spherisorb, 5q silica (4·250mm) column Mobile Phase : Acetonitrile :0.1% orthophosphoric acid Mobile Phase ratio : 65: 35% v/v Detection wavelength :245nm Flow rate : 1ml/min Sample Volume : 20 ql Observation: Using the optimized chromatographic conditions, resolved sharp peaks corresponding to Atorvastatin Calcium and Clopidogrel Bisulphate can be obtained at retention time of 2.007 and 5.977 min respectively.
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.5958/0974-360X.2020.00226.7