Stimulations inducing tethered lipid bilayer formation on protein‐modified substrate

Supported lipid bilayer (SLB) is one artificial biomembrane system used to study transportation reactions through biomembranes using solid devices and surface scientific methods. A key step in the SLB formation is the transformation of spherical lipid vesicles to a planar membrane. We investigated s...

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Bibliographic Details
Published in:Electronics and communications in Japan 2020-09, Vol.103 (9), p.36-42
Main Authors: Tero, Ryugo, Sato, Junichi
Format: Article
Language:English
Subjects:
Calcium ions
fluorescence microscopy
lipid bilayer membrane
Lipids
Silicon dioxide
Silicon substrates
surface chemical modification
vesicle fusion
Vesicles
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Summary:Supported lipid bilayer (SLB) is one artificial biomembrane system used to study transportation reactions through biomembranes using solid devices and surface scientific methods. A key step in the SLB formation is the transformation of spherical lipid vesicles to a planar membrane. We investigated stimulation methods for adsorbed lipid vesicles on a streptavidin‐modified SiO2/Si substrate to induce tethered‐type SLB. We describe the effects of osmotic stress, Ca2+ addition, and a freeze–thaw cycle on the adsorbed vesicles, and on the condition of t‐SLB.
ISSN:1942-9533
1942-9541
DOI:10.1002/ecj.12262