Preparation of 2D coatings of functionally graded chitosan-gold nanocomposite through in-situ reduction in cationic and anionic environments: Application for inhibiting hepatocellular carcinoma cells response

• Published in: Materials chemistry and physics 2020-03, Vol.243, p.122663, Article 122663
• Main Authors: Jaiswal, Juhi, Dhayal, Marshal
• Format: Article
• Language: English
• Subjects: 2D surfaces; Cations; Chitosan; Coatings; Distribution functions; Gold; Gold chloride; Hydroxyl groups; Image analysis; Liver cancer; Metal polymer network; Nanocomposites; Nanoformulation; Nanoparticles; NMR; Nuclear magnetic resonance; Polymer matrix composites; Reduction; Reduction (metal working); Response time; Spectrum analysis; Time dependence
• ISSN: 0254-0584; 1879-3312
• DOI: 10.1016/j.matchemphys.2020.122663

Time-dependent assessment for the formation of gold nanoparticles (GNP) within chitosan (CS) matrix and their relative change in size and distribution was examined by monitoring the in-situ reduction of metal ions. UV–Vis spectroscopic analysis confirmed obtainability of GNP with tunable size, density and functionality which conversed from binomial to monomial distribution function of size at an increased concentration of CS. Uniform distribution of ~7 nm nanoparticles was confirmed by TEM image analysis of polymer-metal composites at optimum conditions. XRD analysis of chitosan-gold (CS-GNP) composite coatings confirmed the presence of gold nanoparticles having two (111) and (200) crystal planes. Energy dispersive X-ray spectroscopy and 1H NMR were carried out to confirm the incorporation and uniform distribution of GNP within the composite. The reduction of gold chloride by chitosan was successively achieved in both acidic (cationic environment) and basic pH (anionic environment). A relative change in the % transmittance at peaks attributing to amine group (1579 cm−1) and hydroxyl group (3405 cm−1) was observed in the FTIR spectra of CS-GNP indicating the role of these groups in reduction process at different pH environments. These functionally graded films showed decreased viability and proliferation of human hepatocellular carcinoma cells (HepG2) at in-vitro conditions. [Display omitted] •A process developed for cohesive composite metal-polymer coatings.•Effectiveness of chitosan interaction with Au(III) is independent of pH.•Functionally graded distribution of nanoparticles achieved through polymer reduction.•Chitosan-gold nanocomposite inhibiting proliferation of HepG2 cells.