Characterisation of the Pseudomonas savastanoi pv. phaseolicola population found in Eastern Australia associated with halo blight disease in Vigna radiata

This study analysed the phenotypic and genotypic variation among 511 Pseudomonas savastanoi pv. phaseolicola (Psp) isolates, causing halo blight in mungbeans. Collected from symptomatic mungbean ( Vigna radiata ) crops throughout Australia between 2005 and 2018, a total of 352 Psp isolates were phen...

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Bibliographic Details
Published in:Australasian plant pathology 2020-09, Vol.49 (5), p.515-524
Main Authors: Noble, Thomas J., Young, Anthony J., Kelly, Lisa A., Barrerro, Roberto A., Douglas, Colin A., Long, Hao, Williams, Brett, Mundree, Sagadevan
Format: Article
Language:English
Subjects:
Agriculture
Beans
Biomedical and Life Sciences
Blight
Cultivars
Deoxyribonucleic acid
Disease resistance
DNA
Ecology
Entomology
Epidemiology
Gene sequencing
Genes
Genetic screening
Genotypes
Halo blight
Life Sciences
Original Paper
Phenotypic variations
Plant Pathology
Plant Sciences
Population genetics
Pseudomonas
Pseudomonas savastanoi
Vigna radiata
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Summary:This study analysed the phenotypic and genotypic variation among 511 Pseudomonas savastanoi pv. phaseolicola (Psp) isolates, causing halo blight in mungbeans. Collected from symptomatic mungbean ( Vigna radiata ) crops throughout Australia between 2005 and 2018, a total of 352 Psp isolates were phenotypically screened. Our in planta screening against a set of four mungbean cultivars with known susceptible and resistant reactions revealed five distinctive pathotypes. Isolates belonging to pathotype 2 were the most prevalent at 84% and were found to be highly pathogenic towards all tested mungbean genotypes. Genomic variation was investigated for 205 isolates using DNA fingerprints, splitting the halo blight pathogen population into two broad genetic lineages. Further genetic testing for two known avirulence genes, avrPphE and avrPphF , identified the avrPphE gene in all the tested isolates and avrPphF present in all but two. To identify candidate avirulence genes unique to Psp isolates infecting mungbean in Australia, a comparative genomics study was undertaken on the whole-genome sequences of two epidemiologically important Psp isolates, T11544 and K4287. The information presented in this study has the potential to dramatically improve mungbean disease resistance now and into the future.
ISSN:0815-3191
1448-6032
DOI:10.1007/s13313-020-00722-8