Hierarchical Ti3C2 MXene-derived sodium titanate nanoribbons/PEDOT for signal amplified electrochemical immunoassay of prostate specific antigen

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2020-03, Vol.860, p.113869, Article 113869
• Main Authors: Xu, Quan, Xu, Jingkun, Jia, Haiyan, Tian, Qingyun, Liu, Peng, Chen, Shuxian, Cai, Yue, Lu, Xinyu, Duan, Xuemin, Lu, Limin
• Format: Article
• Language: English
• Subjects: Alkalizing; Antibodies; Antigens; Biocompatibility; Biosensors; Electrochemical detection; Electron transfer; Electronic properties; Gold; Immunoassay; Immunosensor; Immunosensors; MXene; MXenes; Nanoparticles; Nanoribbons; Oxidation; Poly(3,4-ethylenedioxythiophene); Prostate; Prostate specific antigen; Selectivity; Sodium titanate; Three-dimensional sodium titanate
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2020.113869

Two-dimensional (2D) MXenes have emerged as an attractive platform to construct electrochemical biosensors because of their excellent electronic properties. However, the self-restacking and agglomeration of MXenes lead to unsatisfactory performance. To overcome these drawbacks, herein, the treatment of Ti3C2 MXene through a simultaneous oxidation and alkalization progress was proposed for the synthesis of 3D sodium titanate nanoribbons (M-NTO). Furthermore, conductive poly(3,4-ethyl-enedioxythiophene) (PEDOT) was anchored on 3D M-NTO to enhance its conductivity. The resulting M-NTO-PEDOT composite possessed high specific surface area, fast electron transfer ability, as well as excellent biocompatibility. Subsequently, Au nanoparticles (AuNPs) were electrodeposited on the surface of M-NTO-PEDOT for prostate specific antigen (PSA) antibodies immobilization. Thus, a facile electrochemical label-free immunosensor for sensitive detection of PSA was fabricated on a basis of AuNPs/M-NTO-PEDOT composite. Benefitting from the synergistic effects of M-NTO, PEDOT and AuNPs, the fabricated M-NTO-PEDOT based immunosensor exhibited good analytical performance with a low limit of detection (LOD) of 0.03 pg L−1 (S/N = 3). Additionally, high stability, good selectivity and recovery were achieved in the process of PSA detection in human serum samples. A novel electrochemical immunosensor was fabricated based on M-NTO-PEDOT/AuNPs composite for the detection of Prostate Specific Antigen. [Display omitted] •Hierarchical Ti3C2 MXene-derived sodium titanate nanoribbons (M-NTO)/PEDOT was synthesized.•M-NTO-PEDOT showed high specific surface area, good biocompatibility and conductivity.•A novel immunosensor for PSA detection was fabricated based on AuNPs/M-NTO-PEODT.•AuNPs/M-NTO-PEODT as signal amplifier greatly improved the detection sensitivity.•This immunosensor was successfully applied for the detection of PSA in real serum sample.