Comparison of the osteoblastic activity of low elastic modulus Ti-24Nb-4Zr-8Sn alloy and pure titanium modified by physical and chemical methods

• Published in: Materials Science & Engineering C 2020-08, Vol.113, p.111018, Article 111018
• Main Authors: Zhan, Xinxin, Li, Shujun, Cui, Yuntao, Tao, Anqi, Wang, Chengcheng, Li, Huazhi, Zhang, Linlin, Yu, Hanrong, Jiang, Jiuhui, Li, Cuiying
• Format: Article
• Language: English
• Subjects: Alkaline phosphatase; Alloying elements; Alloys - chemistry; Alloys - pharmacology; Amine oxidase (flavin-containing); Anodic oxidation; Anodizing; Bioactivity of bone marrow mesenchymal stem cells; Biocompatibility; Biological activity; Biomedical materials; Bone marrow; Cell Differentiation - drug effects; Cell proliferation; Cell Proliferation - drug effects; Core Binding Factor Alpha 1 Subunit - metabolism; Cytoskeleton; Cytoskeleton - drug effects; Differentiation (biology); Elastic Modulus; Electrochemistry; Humans; Implantation; Low elastic modulus Ti-24Nb-4Zr-8Sn alloy; Materials science; Mechanical properties; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Mesenchyme; Micro-arc oxidation; Mineralization; Modulus of elasticity; Nodules; Osteoblasts; Osteogenesis; Osteogenesis - drug effects; Oxidation; Oxidation-Reduction; Sandblasted, large-grit, acid-etched; Stem cells; Surface Properties; Titanium; Titanium - chemistry; Titanium - pharmacology; Titanium alloys; Titanium base alloys; Vinculin - metabolism
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2020.111018

Ti-24Nb-4Zr-8Sn (Ti2448) alloy is a novel low elastic modulus β-titanium alloy without toxic elements. It also has the advantage of high strength, so it has potential application prospects for implantation. To develop its osteogenic effects, it can be modified by electrochemical, and physical processes. The main research aim of this study was to explore the bioactivity of Ti2448 alloy modified by sandblasted, large-grit, acid-etched (SLA), micro-arc oxidation (MAO) and anodic oxidation (AO), and to determine which of the three surface modifications is the best way for developing the osteogenesis of bone marrow mesenchymal stem cells (BMMSCs). In vitro studies, the cytoskeleton, focal adhesion and proliferation of BMMSCs showed that both pure titanium and Ti2448 alloy have good biocompatibility. The osteogenic differentiation of BMMSCs with the Ti2448 alloy were examined by detecting alkaline phosphatase (ALP), mineralization nodules and osteogenic proteins and were better than that with pure titanium. These results showed that the Ti2448 alloy treated by SLA has a better effect on osteogenesis than pure titanium, and AO is the best way of three surface treatments to improve osteogenesis in this study. •Microstructure were formed on the surfaces of pure titanium and Ti-24Nb-4Zr-8Sn alloy by surface modifications.•The increase of roughness may be not conducive to cell growth, but was favorable for cell differentiation.•Ti2448 alloy after surface modification has better osteoblast bioactivity than pure titanium.•Anodic oxidation is the best method of three surface modifications to further improve bone formation in vitro.