Specific detection and quantification of Ralstonia pseudosolanacearum race 4 strains from Zingiberaceae plant cultivation soil by MPN-PCR

The most probable number-polymerase chain reaction (MPN-PCR) method was assessed for the specific detection and quantification of Ralstonia pseudosolanacearum race 4 strains in soil from Zingiberaceae fields in Japan. Based on the genome sequence analysis of two bacterial strains [MAFF 211479 (type...

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Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2020-09, Vol.86 (5), p.393-400
Main Authors: Horita, Mitsuo, Sakai, Yoriko K.
Format: Article
Language:English
Subjects:
Agriculture
Bacterial and Phytoplasma Diseases
Biomedical and Life Sciences
Cultivation
Genomes
Ginger
Life Sciences
Microbiology
Most probable number
Nucleotide sequence
Plant Pathology
Polymerase chain reaction
Ralstonia pseudosolanacearum
Sequence analysis
Soils
Strains (organisms)
Zingiberaceae
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Summary:The most probable number-polymerase chain reaction (MPN-PCR) method was assessed for the specific detection and quantification of Ralstonia pseudosolanacearum race 4 strains in soil from Zingiberaceae fields in Japan. Based on the genome sequence analysis of two bacterial strains [MAFF 211479 (type I) and MAFF 211472 (type II)] isolated from ginger, race 4 type I- and type II-specific nested PCR primer sets were designed, respectively. The MPN-PCR using these primer sets efficiently detected and quantified the pathogen in naturally and artificially infested soils.
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-020-00939-x