Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action

Propose: We evaluated the antibacterial potential of the crude leaf extract (CLE) and fractions hexane (HX) and ethyl acetate (EtOAc) from Talinum paniculatum alone and in association with oxacillin (OXA) against OXA-resistant Staphylococcus aureus (ORSA, environment isolates) and OXA-sensitive S. a...

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Published in:Revista colombiana de ciencias químico-farmacéuticas 2020-05, Vol.49 (2)
Main Authors: Cerdeira, Cláudio Daniel, Da Silva, Jeferson J., R. Netto, Manoel F., G. Boriollo, Marcelo F., Santos, Gérsika B., C. dos Reis, Luis F., P. L. Brigagão, Maísa R.
Format: Article
Language:English
Subjects:
Antibiotics
Antimicrobial agents
Biocompatibility
Biological activity
Ethyl acetate
Evaluation
In vivo methods and tests
Penicillin
Staphylococcus infections
Toxicity testing
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Summary:Propose: We evaluated the antibacterial potential of the crude leaf extract (CLE) and fractions hexane (HX) and ethyl acetate (EtOAc) from Talinum paniculatum alone and in association with oxacillin (OXA) against OXA-resistant Staphylococcus aureus (ORSA, environment isolates) and OXA-sensitive S. aureus (OSSA, ATCC 25923). Furthermore, toxicity tests were performed. Methods: The antibacterial activity was evaluated through checkerboard assay (broth microdilution) to establish the minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC). Toxicity test in mice was assessed. Results: The MIC values for the CLE and its fractions against ORSA and OSSA were in the order of HX (500 μg ml–1) = EtOAc < CLE (4000 μg ml–1). EtOAc and HX presented outstanding antibacterial activities against ORSA, and these fractions were bactericidal toward OSSA. Conversely, the associations between plant product (CLE, EtOAc, or HX) and OXA exhibited no synergistic effects. During these associations, there was an increase in OXA MICs anywhere from 2- to 4092-fold. The CLE presented absence of toxicity at a dose of 5 g kg-1 (in vivo). Conclusion: Although T. paniculatum be a good source of bioactive compounds with antistaphylococcal potential, the researchers should be cautious, since its edible leaf may interfere with OXA therapy (mitigating OXA-induced growth inhibition or killing of S. aureus and enhancing S. aureus resistance).
ISSN:0034-7418
1909-6356
DOI:10.15446/rcciquifa.v49n2.89894