Injectable photo-crosslinking cartilage decellularized extracellular matrix for cartilage tissue regeneration

•Cartilage decellularized matrix was processed into hydrogel (CDEM-pHA).•CDEM-pHA was injectable and photo-crosslinkable.•CDEM-pHA combined with chondrocytes formed mature cartilage in vivo. Cartilage decellularized extracellular matrix (CDEM) is a promising biomaterial for preparing tissue engineer...

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Bibliographic Details
Published in:Materials letters 2020-06, Vol.268, p.127609, Article 127609
Main Authors: Xu, Yong, Jia, Litao, Wang, Zongxin, Jiang, Gening, Zhou, Guangdong, Chen, Weiming, Chen, Ru
Format: Article
Language:English
Subjects:
Biomaterials
Biomedical materials
Cartilage
Crosslinking
Decellularized extracellular matrix
Extracellular matrix
Hyaluronic acid
Hydrogels
In vivo methods and tests
Materials science
Photo-crosslinking
Regeneration
Rheological properties
Shear thinning (liquids)
Sol-gel preparation
Storage modulus
Surgical implants
Tissue engineering
Ultraviolet radiation
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Summary:•Cartilage decellularized matrix was processed into hydrogel (CDEM-pHA).•CDEM-pHA was injectable and photo-crosslinkable.•CDEM-pHA combined with chondrocytes formed mature cartilage in vivo. Cartilage decellularized extracellular matrix (CDEM) is a promising biomaterial for preparing tissue engineering scaffolds, and CDEM-based hydrogels constitute an ideal choice for repairing cartilage damage. In this study, CDEM powders and photo-responsive hyaluronic acid (pHA) were successfully fabricated into injectable and photo-crosslinkable hydrogel (CDEM-pHA). The rheological properties of CDEM-pHA indicated that CDEM-pHA exhibited shear-thinning behavior, and the storage modulus of the hydrogel increased with increasing UV irradiation. Chondrocytes were loaded into CDEM-pHA and implanted into the subcutaneous tissue of nude mice for eight weeks; the results indicated that CDEM-pHA combined with chondrocytes successfully regenerated mature cartilage in vivo. This study established a novel strategy for preparing CDEM-based hydrogel for cartilage regeneration.
ISSN:0167-577X
1873-4979
DOI:10.1016/j.matlet.2020.127609