Aluminium triggers oxidative stress and antioxidant response in the microaleae Scenedesmus sp

• Published in: Journal of plant physiology 2020-03, Vol.246-247, p.1
• Main Authors: Ameri, Maryam, Baron-Sola, Angel, Khavari-Nejada, Ramazan Ali, Soltani, Neda, Najafi, Farzaneh, Bagheri, Abdolreza, Martinez, Flor, Hernández, Luis E
• Format: Article
• Language: English
• Subjects: Algae; Aluminum; Antioxidants; Aquatic ecosystems; Ascorbic acid; Biomarkers; Catalase; Cell aggregation; Contamination; Depletion; Enzymatic activity; Fluorescent indicators; Glutathione; Glutathione reductase; Isoforms; L-Ascorbate peroxidase; Lipid peroxidation; Lipids; Oxidation; Oxidative stress; Peroxidase; Peroxidation; Post-translation; Proteins; Reductases; Scenedesmus; Superoxide dismutase; Toxicity; Water pollution
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2020.153114

Aluminium (Al) water pollution is an increasing environmental problem and comprehensive analysis of toxic responses of aquatic primary producer organisms is imperative. We characterized the antioxidant response of Scenedesmus sp. microalga to Al-induced oxidative stress. After 72 h of exposure to Al (0, 10, and 100 µM) in a modified Bold Basal Medium (pH 5.0), we observed cell aggregation and alterations in the subcellular structure, strong lipid peroxidation and oxidative stress induction (detected with the fluorescent probe 2',7'-di-chlorodihydrofluorescein diacetate) in parallel with Al accumulation in cells. At the same time, Al toxicity caused depletion of important macronutrients like Ca, which is important for cell-wall structure. Analysis of antioxidant enzymatic activities in Al-treated Scenedesmus cells revealed that catalase, ascorbate peroxidase, as well as different isoforms of superoxide dismutase were inhibited especially at the highest Al dose (100 µM), cells that accumulated the highest concentration of Al. On the other hand, glutathione reductase activity increased at that Al concentration. Immunodetection after Western-blotting confirmed that only ascorbate peroxidase inhibition was apparently due to a decrease in enzyme levels. However, the inhibition of catalase and activation of glutathione reductase activities seemed related with post-translational modifications in protein function as protein expression decreased or increased, respectively under Al stress. Our results may help to understand toxic mechanisms triggered by Al in freshwater microalgae, which in turn could aid to select suitable biomarkers of Al contamination in aquatic ecosystems.