Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N′-methylene-bis-acrylamide)-clay based pH/temperature-responsive nanocomposites as drug-carriers

• Published in: Materials Science & Engineering C 2020-05, Vol.110, p.110628, Article 110628
• Main Authors: Bera, Hriday, Abbasi, Yasir Faraz, Gajbhiye, Virendra, Liew, Kok Fui, Kumar, Pramod, Tambe, Prajakta, Azad, A.K., Cun, Dongmei, Yang, Mingshi
• Format: Article
• Language: English
• Subjects: A549 Cells; Acrylamide; Acrylamides - chemistry; Acrylamides - pharmacokinetics; Acrylamides - pharmacology; Apoptosis; Apoptosis - drug effects; Bentonite; Biodegradability; Biodegradation; Cell growth; Cell proliferation; Cell Proliferation - drug effects; Crosslinking; Current carriers; Cytotoxicity; Delayed-Action Preparations - chemistry; Delayed-Action Preparations - pharmacokinetics; Delayed-Action Preparations - pharmacology; Drug carriers; Drug Carriers - chemistry; Drug Carriers - pharmacokinetics; Drug Carriers - pharmacology; Drug delivery; Elution; Fenugreek; Fenugreek galactomannan; Hot Temperature; Humans; Hydrogen-Ion Concentration; Isopropylacrylamide; Lung cancer; Lung Neoplasms - drug therapy; Lung Neoplasms - metabolism; Mannans - chemistry; Mannans - pharmacokinetics; Mannans - pharmacology; Materials science; Methylene bisacrylamide; Models, Chemical; Mucin; Nanocomposites; pH effects; Tailor-made biopolymer; Temperature dependence; Toxicity testing; Trigonella foenum-graecum; Zeta potential
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2020.110628

The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N′-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation. The blank NCs exhibited outstanding biodegradability and pH/temperature-dependent swelling profiles, which were significantly influenced by their BEN contents (0–20%). The molar mass (M¯c) between the crosslinks of these NCs was declined with temperature. The composite architecture of these scaffolds was confirmed by XRD, FTIR, TGA, DSC and SEM analyses. The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93–100%) with zeta potential between −8 and −16 mV and diameter between 615 and 1258 nm. These formulations demonstrated sustained ERL elution profiles (Q8h, 62–98%) with an initial burst release of drug. The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion. The mucin adsorption behavior of F-3 was best fitted to Freudlich isotherms. The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay. [Display omitted] •CFG-g-P(NIPA-co-MBA)-BEN based responsive NCs was synthesized.•It portrayed pH/temperature-dependent swelling and outstanding biodegradability.•ERL was loaded via self-assembly technique assisted by ultrasonication.•Formulation F-3 displayed highest DEE with prolonged drug release at 8 h.•It effectively suppressed A549 cell growth and promoted apoptosis.