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Effect of Viral Infection on the Functioning and Lysis of Black Sea Microalgae Tetraselmis viridis (Chlorophyta) and Phaeodactylum tricornutum (Bacillariophyta)
Experimental studies have been performed on Tetraselmis viridis (Rouchijajnen) R.E. Norris, Hori & Chihara (Chlorophyta) and Phaeodactylum tricornutum Bohlin (Bacillariophyta) cultures of Black Sea microalgae using TsV-S1 and PtV-S18 algal virus strains isolated from the Black Sea ecosystem. The...
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Published in: | Inland water biology 2020-07, Vol.13 (3), p.417-424 |
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Main Authors: | , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Experimental studies have been performed on
Tetraselmis viridis
(Rouchijajnen) R.E. Norris, Hori & Chihara (Chlorophyta) and
Phaeodactylum tricornutum
Bohlin (Bacillariophyta) cultures of Black Sea microalgae using TsV-S1 and PtV-S18 algal virus strains isolated from the Black Sea ecosystem. The study assesses the effects of light intensity and the initial abundance of microalgae cells on the onset of their infection by viruses and the decline in their abundance. As early as on the second or third day, the cells changed their shape and increased in volume in the cultures infected by viruses. At this time, a decrease was observed in chlorophyll
a
red autofluorescence, variable fluorescence per cell, and photochemical efficiency of photosystem 2. In the studied cultures, a reduction in cell abundance due to viral lysis was noted 1 to 2 days later. The threshold cell abundance at which this process was observed was independent of light conditions and amounted to 3 × 10
5
cells/mL for
T. viridis
and 18 × 10
5
cells/mL for
Ph. tricornutum
. The complete lysis of algae cells was detected by the end of the fourth to sixth day. In the dark, the effect of viruses on microalgae was insignificant. |
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ISSN: | 1995-0829 1995-0837 |
DOI: | 10.1134/S1995082920020303 |