Cell viability in sh-sy5y neuroblastoma cells in folic acid application by using jack-stat pathway

• Published in: Gülhane tıp dergisi 2019-12, Vol.61 (4), p.157
• Main Authors: Guran, Sefik, Kaplan, Zehra Zeynep, Kilic, Yigit Cemo, Coban, Zehra Dilcad, Polat, Yunus Emre, Gulec, Kardelen, Yuce, Alpertunga, Tuncbilek, Vildan, Baykara, Meral Zehra, Kilicarslan, Ozgur
• Format: Article
• Language: English
• Subjects: Acids; Cell growth; Folic acid; Gene expression; Genes; Genetic research; Neuroblastoma; Pregnancy; Vitamin B
• ISSN: 2146-8052; 1302-0471; 2146-8052
• DOI: 10.26657/gulhane.00076

Aims: Folic acid and folate are forms of a water-soluble B vitamin. Folic acid is used as a supplement by women during pregnancy to reduce the risk of neural tube defects. Folic acid supplementation is reported with the suppression of tumor development in literature. Here, the effect of folic acid was analyzed for cell proliferation and viability on neuroblastoma cells. The gene expression differences of folic acid receptors and JAK/STAT pathway were analyzed. Methods: FA solutions were prepared on 1 [micro]M, 5 [micro]M, 10 [micro]M concentrations and applied on neuroblastoma SH-SY5Y cells. XTT cell proliferation assay and cell viability assay were used for finding cell proliferation and viability. The gene expression differences was analyzed on FOLR1, JAK1, STAT3, PIAS1, PTPN1 and SOCS-1 genes on neuroblastoma cells by using real time polymerase chain reaction. Results: In XTT assay, LD50 dosage was found as 22 [micro]M FA concentration on SH-SY5Y neuroblastoma cells. Cell viability was found as 93% in control, 96% in 1-5 [micro]M, 97% in 10 [micro]M folic acid application (p