Assessing the effect of chitosan on pesticide removal in grape juice during clarification by gas chromatography with tandem mass spectrometry

• Published in: Process biochemistry (1991) 2020-07, Vol.94, p.305-312
• Main Authors: Venkatachalapathy, Renuka, Packirisamy, Azhagu Saravana Babu, Indira Ramachandran, Anoop Chandra, Udhyasooriyan, Lalitha Priya, Peter, Maria Jenita, Senthilnathan, Karthikeyan, Basheer, Vajiha Aafrin, Muthusamy, Sukumar
• Format: Article
• Language: English
• Subjects: Activated carbon; Bentonite; Casein; Chitosan; Chlorpyrifos; Chromatography; clarification; Diazinon; Fenitrothion; Fenthion; Fruit juices; Fungicides; Gas chromatography; GC-MS/MS; Gelatin; Grapes; incubation; Insecticides; Juices; Mass spectrometry; Mass spectroscopy; pesticide removal; Pesticides; Phorate; Pollutant removal; Scientific imaging; Spectroscopy
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2020.04.035

[Display omitted] •Pesticide removal efficiency of chitosan in grape juice was investigated.•Multi residue analysis of six pesticides was applied.•Four other clarifying agents were studied for its pesticide removal efficiency.•Pesticide removal efficiency of chitosan was higher than other clarifiers.•The pesticide removal was correlated with its hydrophobicity. The main objective of this study was to assess the pesticide removal efficiency of chitosan in grape juice during clarification. The grape juice was spiked with six selected pesticides (chlorpyrifos, ethion, diazinon, fenitrothion, fenthion and phorate) and clarified with chitosan at different concentrations and at three different incubation times (1, 2 and 4 h). The effect of chitosan on the level of pesticides in grape juice during clarification was quantified using modified QuEChERS method with gas chromatography with tandem mass spectrometry (GC-MS/MS). The method for pesticide quantification has been validated. The maximum removal was obtained for chlorpyrifos (98%) and ethion (97%) at 0.5% of chitosan concentration for 1 h incubation followed by phorate (96%), fenthion (95%), fenitrothion (94%) and diazinon (86%) at 1% of chitosan concentration for 2 h incubation time. The juice was clarified with other clarifying agents such as activated carbon, gelatin, casein and bentonite and its pesticide removal efficiency was compared with chitosan. The pesticide removal level could be correlated with the properties of selected pesticides and clarifying agents. The results indicated that the chitosan can act as an effective pesticide scavenger and it leads to the potential application of chitosan in fruit juice clarification.