Effects of the cross-linker on the performance and stability of enzymatic electrocatalytic films of glucose oxidase and dimethylferrocene-modified linear poly(ethyleneimine)

This work compares the performance of bioelectrocatalytic films composed of glucose oxidase, dimethylferrocene-modified linear poly(ethyleneimine) and either glutaraldehyde (GA) or ethylene glycol diglycidyl ether (EGDGE) as cross-linking agents. While GA has been widely employed as an enzyme cross-...

Full description

Saved in:
Bibliographic Details
Published in:Electrochimica acta 2020-03, Vol.337, p.135782, Article 135782
Main Authors: Díaz-González, Jan-carlo M., Escalona-Villalpando, Ricardo A., Arriaga, Luis G., Minteer, Shelley D., Casanova-Moreno, Jannu R.
Format: Article
Language:English
Subjects:
Biofuel cell
Biosensors
cross-linker
Crosslinking
Enzymes
Ethylene glycol diglycidyl ether
Fuel cells
Glucose biosensing
Glucose oxidase
Glutaraldehyde
Hydrogels
Microfluidics
Oxidation
Polyethyleneimine
Stability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This work compares the performance of bioelectrocatalytic films composed of glucose oxidase, dimethylferrocene-modified linear poly(ethyleneimine) and either glutaraldehyde (GA) or ethylene glycol diglycidyl ether (EGDGE) as cross-linking agents. While GA has been widely employed as an enzyme cross-linker, its use with mediator-modified linear poly(ethyleneimine)s has not been reported. Here, we evaluate both types of polymers for use in a drop-based biosensor and a microfluidic fuel cell. In the sensing context, GA-based polymers presented higher stability at the expense of linear response range. In the energy production device, significant differences in current were not observed when comparing the performance of complete cells. We conclude that glutaraldehyde is a suitable alternative to the widespread epoxide cross-linkers for the fabrication of enzyme-containing ferrocene-modified linear poly(ethyleneimine) hydrogels. [Display omitted]
ISSN:0013-4686
1873-3859
DOI:10.1016/j.electacta.2020.135782