An Affinity-Enhanced DNA Intercalator with Intense ECL Embedded in DNA Hydrogel for Biosensing Applications

Herein, an amphiphilic perylene derivative (denoted as PTC-DEDA) was explored as DNA intercalators endowed with an enhanced affinity and intense electrochemiluminescence (ECL) to construct a target-induced DNA hydrogel biosensing platform for the sensitive detection of microRNA let-7a (miRNA let-7a)...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-08, Vol.92 (16), p.11044-11052
Main Authors: Zhao, Mei-Ling, Zeng, Wei-Jia, Chai, Ya-Qin, Yuan, Ruo, Zhuo, Ying
Format: Article
Language:English
Subjects:
Affinity
Biochemical analysis
Biosensing Techniques - methods
Biosensors
Cell Line, Tumor
Chemistry
Dendritic structure
Deoxyribonucleic acid
DNA
DNA - chemistry
Electrochemiluminescence
Emitters
Gold - chemistry
Grooves
Humans
Hydrogels
Hydrogels - chemistry
Intercalating Agents - chemistry
Limit of Detection
Luminescent Agents - chemistry
Magnetite Nanoparticles - chemistry
MicroRNAs
MicroRNAs - analysis
miRNA
Palladium - chemistry
Perylene - analogs & derivatives
Ribonucleic acid
RNA
Sulfates - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Herein, an amphiphilic perylene derivative (denoted as PTC-DEDA) was explored as DNA intercalators endowed with an enhanced affinity and intense electrochemiluminescence (ECL) to construct a target-induced DNA hydrogel biosensing platform for the sensitive detection of microRNA let-7a (miRNA let-7a). Specifically, the DNA hydrogel with numerous dendritic DNA structures was in situ generated via a target-induced nonlinear hybrid chain reaction in the presence of miRNA let-7a, which possessed a large loading capacity to entrap massive DNA intercalators. Then, the PTC-DEDA with positive charges could easily intercalate into the DNA grooves due to the inherent amphipathic structure, achieving a strong ECL signal. Using the proposed PTC-DEDA as both DNA intercalators and ECL emitters, the DNA hydrogel biosensing platform exhibited a high stability and an excellent sensitivity for miRNA let-7a, with a desirable linear range (10 fM to 10 nM) and a low detection limit (1.49 fM). Significantly, the work provides a potential alternative to develop simple and high-efficiency ECL platforms for biochemical analysis applications.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c00152