A novel lysosome targeted fluorophore for H2S sensing: Enhancing the quantitative detection with successive reaction sites

[Display omitted] •A lysosome targeting fluorophore Lyso-Rh-S-DNP has been designed with two reaction sites for H2S sensing.•Enhanced forming three linear relationships between the probe dosage and H2S concentration.•The probe performs short response time, high selectivity and low LOD value towards...

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Published in:Sensors and actuators. B, Chemical Chemical, 2020-10, Vol.320, p.128433, Article 128433
Main Authors: Muthusamy, Selvaraj, Rajalakshmi, Kanagaraj, Zhu, Dongwei, Zhao, Long, Wang, Shengjun, Zhu, Weihua
Format: Article
Language:English
Subjects:
Amides
Emission
Ether cleavage
Fluorescence
Fluorophore
Hydrogen sulfide
Lysosome targeting
Medical imaging
NMR
Nuclear magnetic resonance
Quantitative detection of H2S
Rhodamine
Ring opening
Selectivity
Spiro-lactum ring opening
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Summary:[Display omitted] •A lysosome targeting fluorophore Lyso-Rh-S-DNP has been designed with two reaction sites for H2S sensing.•Enhanced forming three linear relationships between the probe dosage and H2S concentration.•The probe performs short response time, high selectivity and low LOD value towards H2S in biosystems.•Lyso-Rh-S-DNP shows excellent biocompatibility in Hela cells and zebra fish. A novel lysosome targeting fluorophore Lyso-Rh-S-DNP was designed with integration of electron withdrawing 2,4-dintrophenyl (DNP) and electron donating salicylaldehyde (S) on rhodamine (Rh) backbone for unusual successive reaction based emission leading to the quantitative detection of H2S. The emission intensity was shifted linearly with the introduction of three individual range of H2S. The distinguishable fluorescent behavior was ascribed to the sequence of ether (C-O) cleavage and Spiro-lactam ring opening, which was verified using NMR and HR-MS techniques. In addition, the probe expressed fast response (3 min.), high selectivity and remarkably low detection limit (8 nM) towards H2S. A series of successful confocal scans in Hela cells and zebra fish showing H2S concentration dependent bioimaging (cyan, green and red) further demonstrated the applicability of this fluorophore for predicting cancer in early stages in bio-systems.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2020.128433