Synthesis of a Two-Layer Polyionic Bienzyme Complex of Superoxide Dismutase 1 and Catalase of the Smallest Size

We study the factors affecting the size of two-layer polyionic bienzyme SOD1/catalase conjugates: the nature and concentration of the buffer solution, the pH maintained during particle synthesis, and the initial molar ratio of the enzymes. The optimal conditions for obtaining conjugates of the minim...

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Bibliographic Details
Published in:Moscow University chemistry bulletin 2020-07, Vol.75 (4), p.238-242
Main Authors: Tagirova, M. A., Eremeev, N. L., Vaneev, A. N., Balabushevich, N. G., Aleksashkin, A. D., Zaitseva, E. A., Klyachko, N. L.
Format: Article
Language:English
Subjects:
Buffer solutions
Catalase
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Conjugates
Enzymes
Storage stability
Superoxide dismutase
Synthesis
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Summary:We study the factors affecting the size of two-layer polyionic bienzyme SOD1/catalase conjugates: the nature and concentration of the buffer solution, the pH maintained during particle synthesis, and the initial molar ratio of the enzymes. The optimal conditions for obtaining conjugates of the minimum size are determined. The conjugates obtained under these conditions demonstrate the high storage stability of both enzymes.
ISSN:0027-1314
1935-0260
DOI:10.3103/S0027131420040082