Extruded polysaccharide/protein matrix from Arthrospira platensis cultures mediated silver nanoparticles biosynthesis and capping

Herein, Silver nanoparticles (AgNPs) have been synthesized and capped using secreted polysaccharide-protein matrix (EPS) from Arthrospira platensis cultures ( A -EPS) and compared with EPS extracted from 1.0 K Gy gamma-irradiated A. platensis cultures (I A -EPS). Gamma irradiation enhanced EPS secre...

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Bibliographic Details
Published in:Applied nanoscience 2020, Vol.10 (10), p.3839-3855
Main Authors: Hamouda, Ragaa A., Hussein, Mervat H., Elhadary, Abdel Monsef A., Abuelmagd, Muhammad A.
Format: Article
Language:English
Subjects:
Biosynthesis
Capping
Carbohydrates
Chemistry and Materials Science
Dispersion
Extrusion
Fourier transforms
Gamma irradiation
Infrared analysis
Infrared spectrophotometers
Infrared spectroscopy
Materials Science
Membrane Biology
Nanochemistry
Nanoparticles
Nanotechnology
Nanotechnology and Microengineering
Original Article
Polysaccharides
Proteins
Sheaths
Silver
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Summary:Herein, Silver nanoparticles (AgNPs) have been synthesized and capped using secreted polysaccharide-protein matrix (EPS) from Arthrospira platensis cultures ( A -EPS) and compared with EPS extracted from 1.0 K Gy gamma-irradiated A. platensis cultures (I A -EPS). Gamma irradiation enhanced EPS secretion and modified its biochemical composition. A. platensis cultures have been exposed to two acute gamma irradiation doses of 1.0–2.0 K Gy, EPS secretion was scaled up in response to gamma irradiation from 0.559 g L −1 at non-irradiated culture to 0.752, 0.690 g L −1 at 1.0–2.0 K Gy, respectively. Saccharide-protein nature of EPSs was confirmed via chemical, UV Visible spectrophotometer, and Fourier-transform infrared spectroscopy (FT-IR), revealed an increment of protein content in (I A -EPS). Hetero-polymeric constituents were elucidated using HPLC analysis which revealed seven monomers in the saccharide portion of (I A -EPS). AgNP biosynthesis was fabricated in alkaline medium whereas I A -EPS-mediated AgNPs (IEPS-AgNPs) exhibited a stronger UV absorbance than A -EPS-mediated AgNPs (EPS-AgNPs) at pH 9.0 and 2.0 mg L −1 . Synthesized AgNPs displayed the difference in coating nature and its aggregation under Transmission Electron Microscope (TEM) whereas (IEPS-AgNPs) displayed mono-dispersed nanoparticles capped by protein layers meanwhile (EPS-AgNPs) exhibited poly-dispersed ones capped with a faint sheath of EPS. FT-IR analysis illustrated the capping mechanism for both EPS-AgNPs and IEPS-AgNPs.
ISSN:2190-5509
2190-5517
DOI:10.1007/s13204-020-01490-z