Poly(vinylidene) fluoride membranes coated by heparin/collagen layer-by-layer, smart biomimetic approaches for mesenchymal stem cell culture

• Published in: Materials Science & Engineering C 2020-12, Vol.117, p.111281, Article 111281
• Main Authors: Guillot-Ferriols, M., Rodríguez-Hernández, J.C., Correia, D.M., Carabineiro, S.A.C., Lanceros-Méndez, S., Gómez Ribelles, J.L., Gallego Ferrer, G.
• Format: Article
• Language: English
• Subjects: Beta phase; Biomedical materials; Biomimetic materials; Biomimetics; Cell culture; Cell proliferation; Coatings; Cobalt; Cobalt ferrites; Collagen; Differentiation (biology); Fluorides; Heparin; Humans; Layer-by-layer; Magnetic fields; Magnetostriction; Materials science; Membranes; Mesenchymal Stem Cells; Nanoparticles; Ninhydrin; Non-solvent induced phase separation; Nuclei (cytology); Osteogenesis; Phase separation; Photoelectron spectroscopy; Photoelectrons; Piezoelectricity; Poly(vinylidene) fluoride; Polyelectrolytes; Polymers; Polyvinyls; Stem cells; Surface charge; Tissue engineering; Vinylidene; X ray photoelectron spectroscopy
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2020.111281

The use of piezoelectric materials in tissue engineering has grown considerably since inherent bone piezoelectricity was discovered. Combinations of piezoelectric polymers with magnetostrictive nanoparticles (MNP) can be used to magnetoelectrically stimulate cells by applying an external magnetic field which deforms the magnetostrictive nanoparticles in the polymer matrix, deforming the polymer itself, which varies the surface charge due to the piezoelectric effect. Poly(vinylidene) fluoride (PVDF) is the piezoelectric polymer with the largest piezoelectric coefficients, being a perfect candidate for osteogenic differentiation. As a first approach, in this paper, we propose PVDF membranes containing magnetostrictive nanoparticles and a biomimetic heparin/collagen layer-by-layer (LbL) coating for mesenchymal stem cell culture. PVDF membranes 20% (w/v) with and without cobalt ferrite oxide (PVDF-CFO) 10% (w/w) were produced by non-solvent induced phase separation (NIPS). These membranes were found to be asymmetric, with a smooth surface, crystallinity ranging from 65% to 61%, and an electroactive β-phase content of 51.8% and 55.6% for PVDF and PVDF-CFO, respectively. Amine groups were grafted onto the membrane surface by an alkali treatment, confirmed by ninhydrin test and X-ray photoelectron spectroscopy (XPS), providing positive charges for the assembly of heparin/collagen layers by the LbL technique. Five layers of each polyelectrolyte were deposited, ending with collagen. Human mesenchymal stem cells (hMSC) were used to test cell response in a short-term culture (1, 3 and 7 days). Nucleus cell counting showed that LbL favored cell proliferation in PVDF-CFO over non-coated membranes. [Display omitted] •Non-induced phase separation is a reliable technique for PVDF membrane synthesis.•Inclusion of cobalt ferrite oxides (CFO) increases β-phase membrane content.•Collagen and heparin were successfully deposited by layer-by-layer on the membranes.•Layer-by-layer enhances mesenchymal stem cell proliferation on PVDF-CFO membranes.