Evaluation of pathogenicity of indigenous entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from Mizoram, India, against tobacco cutworm, Spodoptera litura (Fabricius, 1775)

Aim: To evaluate pathogenicity of locally isolated entomopathogenic nematodes Heterorhabditis indica, Heterorhabditis baujardi and Steinernema sangi against larvae of S. litura (Lepidoptera:Noctuidae). Methodology: Different concentrations of nematodes (10, 25, 50, 100, 200, 400 and 800 infective ju...

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Bibliographic Details
Published in:Journal of environmental biology 2020-07, Vol.41 (4), p.851-860
Main Authors: Lalramnghaki, HC, Lalremsanga, HT, Vanramliana, Lalramliana, Vanlalhlimpuia, Lalliansanga, S
Format: Article
Language:English
Subjects:
Agricultural practices
Bacteria
Bioassays
Crops
Entomopathogenic nematodes
Environmental science
Evaluation
Experiments
Inoculation
Insects
Integrated pest management
Juveniles
Larvae
Mortality
Nematodes
Pathogenicity
Pathogens
Pest control
Pesticides
Pests
Regression analysis
Tobacco
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Summary:Aim: To evaluate pathogenicity of locally isolated entomopathogenic nematodes Heterorhabditis indica, Heterorhabditis baujardi and Steinernema sangi against larvae of S. litura (Lepidoptera:Noctuidae). Methodology: Different concentrations of nematodes (10, 25, 50, 100, 200, 400 and 800 infective juveniles per larva) of H. indica, H. baujardi and S. sangi were used against 3rd 4th and 5th instar larvae of S. litura using Petri dish assay. Pathogenicity test was performed by assessing larval mortality of S. litura, penetration of infective juveniles and successive production of nematodes on the larvae of S. litura. LC50 and LT50 values were calculated by Probit Regression Analysis using SPSS software 20.0. Results: All the tested EPN isolates showed high pathogenicity against the pest, with a strong positive correlation between nematode concentrations, inoculation time and larval mortality. Among the isolates, S. sangi showed highest pathogenicity against the pest by showing the lowest value of LC50 at 48 hr post-inoculation (9.2, 55.58 and 350.65 infective juveniles for 3rd, 4th and 5th instar larvae, respectively) and LT50values (24.52, 34.13 and 74.69 hr against 3rd, 4th and 5th instar larvae, respectively) at a dosage of 50 infective juveniles per larva. Besides, the nematodes were produced successfully inside the 5th instar larvae of S. litura (57192.86 infective juveniles per larva for S. sangi and 72231.13 infective juveniles per larva for H. baujardi). Interpretation: EPN isolates represent good candidates to control S. litura and can be considered for integrated pest management (IPM).
ISSN:0254-8704
2394-0379
DOI:10.22438/jeb4(SI)/MS_1905