Hierarchically Porous S/N Codoped Carbon Nanozymes with Enhanced Peroxidase-like Activity for Total Antioxidant Capacity Biosensing

Design of highly active carbon nanozymes and further establishment of ultrasensitive biosensors remain a challenge. Herein, hierarchically porous carbon nanozymes with sulfur (S)/nitrogen (N) codoping (SNC) were developed. Compared with N-doped carbon (NC) nanozymes, SNC nanozymes have a smaller Mic...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-10, Vol.92 (19), p.13518-13524
Main Authors: Chen, Yifeng, Jiao, Lei, Yan, Hongye, Xu, Weiqing, Wu, Yu, Wang, Hengjia, Gu, Wenling, Zhu, Chengzhou
Format: Article
Language:English
Subjects:
Activated carbon
Antioxidants
Antioxidants - analysis
Antioxidants - metabolism
Ascorbic acid
Ascorbic Acid - analysis
Ascorbic Acid - metabolism
Beverages
Biosensing Techniques
Biosensors
Carbon
Carbon - chemistry
Chemistry
Colorimetry
Nanostructures - chemistry
Nitrogen
Nitrogen - chemistry
Particle Size
Peroxidase
Peroxidase - chemistry
Peroxidase - metabolism
Porosity
Selectivity
Substrates
Sulfur
Sulfur - chemistry
Surface Properties
Synergistic effect
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Summary:Design of highly active carbon nanozymes and further establishment of ultrasensitive biosensors remain a challenge. Herein, hierarchically porous carbon nanozymes with sulfur (S)/nitrogen (N) codoping (SNC) were developed. Compared with N-doped carbon (NC) nanozymes, SNC nanozymes have a smaller Michaelis–Menten constant and higher specific activities, demonstrating that the S-doping in SNC nanozymes could not only enhance their affinity toward substrates but also improve their catalytic performance. These results may be caused by the synergistic effect of heteroatoms (S and N). Because of the good enzyme-like activity, the proposed SNC nanozymes were exploited to the colorimetric detection of the total antioxidant capacity (TAC) using ascorbic acid as a typical model with a limit of detection of 0.08 mM. Because of its high sensitivity and selectivity and encouraging performance, the detection method presented practical feasibility for the TAC assay in commercial beverages. This work paves a way to design the highly active carbon nanozymes and expand their applications in the construction of high-performance biosensors.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c02982