Flow, suspension, and mixing dynamics in DASGIP bioreactors: Part 1

The bioreactor flow environment has a significant impact on process performance, especially in stem cell cultures. The work of Correia et al found intermittent agitation modes to improve induced pluripotent stem cell (iPSC)‐cardiomyocyte differentiation yields; however, to date, the impact within th...

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Bibliographic Details
Published in:AIChE journal 2020-11, Vol.66 (11), p.n/a
Main Authors: Samaras, Jasmin J., Micheletti, Martina, Ducci, Andrea
Format: Article
Language:English
Subjects:
Agitation
agitation modes
Axial flow
bioreactor geometry
Bioreactors
Cell differentiation
DASGIP
Differentiation
Environmental impact
flow dynamics
Flow velocity
Impellers
PIV
Pluripotency
Shear stress
Spikes
Stem cells
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Summary:The bioreactor flow environment has a significant impact on process performance, especially in stem cell cultures. The work of Correia et al found intermittent agitation modes to improve induced pluripotent stem cell (iPSC)‐cardiomyocyte differentiation yields; however, to date, the impact within the flow has not been fully characterized. This work aims to characterize the flow dynamics occurring within a commercially available DASGIP bioreactor, equipped with a two‐blade paddle impeller, operating under different agitation modes and for two bottom geometries. The paddle impeller configuration generated an axial flow profile due to a large impeller D/T and blade confinement with the bioreactor wall. The application of intermittent agitation was shown to induce two transient spikes in flow velocity and shear stress, the amplification of which increased with dwell duration. Marginally increasing the dwell duration was shown previously to increase differentiation yields, therefore it can be stipulated that introduction of these spikes was favorable toward cardiogenic differentiation.
ISSN:0001-1541
1547-5905
DOI:10.1002/aic.17014