Evaluation of OptiFlow™-MS/MS for bioanalysis of pharmaceutical drugs and metabolites

Microflow tandem mass spectrometry-based methods have been proposed as options to improve sensitivity and selectivity while improving sample utility and solvent consumption. Here, we evaluate a newly introduced microflow source, OptiFlow™, for quantitative performance. We performed a comparison of t...

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Bibliographic Details
Published in:Bioanalysis 2020-01, Vol.12 (1), p.23-34
Main Authors: Barricklow, Jason, Tweed, Joseph, Holliman, Christopher L, Ramanathan, Ragu
Format: Article
Language:English
Subjects:
Acids
Alprazolam
Chromatography
LC–MS/MS
Mass spectroscopy
Metabolites
microflow
microsampling
Midazolam
Pharmaceutical industry
pharmacokinetics
Plasma
Quantitation
Solvents
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Summary:Microflow tandem mass spectrometry-based methods have been proposed as options to improve sensitivity and selectivity while improving sample utility and solvent consumption. Here, we evaluate a newly introduced microflow source, OptiFlow™, for quantitative performance. We performed a comparison of the OptiFlow and IonDrive™ sources, respectively, on the same triple quadrupole mass spectrometer. The comparison used a neat cocktail of commercially available drugs and extracted plasma samples monitoring midazolam and alprazolam metabolites. Microflow produced a 2–4× signal increase for the neat drug cocktail and a 5–10× increase for extracted plasma samples. The OptiFlow method consistently gave increased signal response relative to the IonDrive method and enabled a better lower limit of quantitation for defining phamacokinetics.
ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2019-0250