Serum Choice Influences Lipid Accumulation and Cell Viability in Fatty Acid Treated Immortalised Hepatocytes and Hepatic Stellate Cells

[...]to test if there was interference from lipophilic materials contained in the serum, the SCM was either replenished, replaced with CSM, or replaced with SFM, and cells cultured for 16hrs in the fresh media prior to 6 h or 24 h fatty acid treatment (0-500μM, 1:1 oleic acid: palmitic acid). [...]n...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the Nutrition Society 2020, Vol.79 (OCE3), Article E751
Main Authors: Zhang, Z., Thorne, J.L., Moore, J.B.
Format: Article
Language:English
Subjects:
Accumulation
Calciferol
Cell lines
Cell viability
Experiments
Fatty acids
Gene expression
Hepatocytes
Lipids
Lipophilic
Liver diseases
miRNA
mRNA
Nutrient loading
Oleic acid
Palmitic acid
Ribonucleic acid
RNA
Stellate cells
Vitamin D
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[...]to test if there was interference from lipophilic materials contained in the serum, the SCM was either replenished, replaced with CSM, or replaced with SFM, and cells cultured for 16hrs in the fresh media prior to 6 h or 24 h fatty acid treatment (0-500μM, 1:1 oleic acid: palmitic acid). [...]no differences in viability were detected between cells cultured in CSM compared to SCM.Fatty acid treatment led to dose-dependent intracellular lipid accumulation in both cell lines, however, effects from serum were observed. Using CSM for vitamin D and fatty acid cotreatment, ongoing experiments are examining mRNA, protein, and microRNA expression in hepatocytes and HSCs in response to vitamin D and lipid loading cotreatment. 1.
ISSN:0029-6651
1475-2719
DOI:10.1017/S0029665120007375