Selective cell encapsulation, lysis, pico-injection and size-controlled droplet generation using traveling surface acoustic waves in a microfluidic device

We generate droplets in a microfluidic device using a traveling surface acoustic wave (TSAW), and control droplet size by adjusting TSAW power and duration. We combine droplet production and fluorescence detection to selectively-encapsulate cells and beads; with this active method, the overwhelming...

Full description

Saved in:
Bibliographic Details
Published in:Lab on a chip 2020-11, Vol.2 (21), p.3914-3921
Main Authors: Mutafopulos, Kirk, Lu, Peter J, Garry, Ryan, Spink, Pascal, Weitz, David A
Format: Article
Language:English
Subjects:
Beads
Droplets
Encapsulation
Fluorescence
Microfluidic devices
Poisson distribution
Surface acoustic waves
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We generate droplets in a microfluidic device using a traveling surface acoustic wave (TSAW), and control droplet size by adjusting TSAW power and duration. We combine droplet production and fluorescence detection to selectively-encapsulate cells and beads; with this active method, the overwhelming majority of single particles or cells are encapsulated individually into droplets, contrasting the Poisson distribution of encapsulation number that governs traditional, passive microfluidic encapsulation. In addition, we lyse cells before selective encapsulation, and pico-inject new materials into existing droplets. We generate traveling surface acoustic waves with an interdigital transducer to create droplets on-demand; encapsulate single cells; lyse cells and immediately encapsulate their contents; and pico-inject new materials into existing droplets.
ISSN:1473-0197
1473-0189
DOI:10.1039/d0lc00723d