Plasma/serum proteomics: depletion strategies for reducing high-abundance proteins for biomarker discovery

Plasma and serum are widely used for proteomics-based biomarker discovery. However, analysis of these biofluids is highly challenging due to the complexity and wide dynamic range of their proteomes. Notably, highly abundant proteins tend to obscure the detection of potential biomarkers that are usua...

Full description

Saved in:
Bibliographic Details
Published in:Bioanalysis 2019-10, Vol.11 (19), p.1799-1812
Main Authors: Lee, Pey Yee, Osman, Junaida, Low, Teck Yew, Jamal, Rahman
Format: Article
Language:English
Subjects:
Antibodies
Apolipoproteins
bacterial cellulose
Binding sites
biomarker
Biomarkers
Chromatography
cibacron blue
combinatorial peptide ligand library (CPLL)
cryogels
depletion
Efficiency
Ligands
Methods
molecular imprinted polymers
Plasma
Proteins
Proteomics
serum
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plasma and serum are widely used for proteomics-based biomarker discovery. However, analysis of these biofluids is highly challenging due to the complexity and wide dynamic range of their proteomes. Notably, highly abundant proteins tend to obscure the detection of potential biomarkers that are usually of lower concentrations. Among the strategies to resolve this problem are: depletion of high-abundance proteins, enrichment of low abundant proteins of interest and prefractionation. In this review, we focus on current and emerging depletion techniques used to enhance the detection and identification of the less abundant proteins in plasma and serum. We discuss the applications and contributions of these methods to proteomics analysis of plasma and serum alongside their limitations and future perspectives.
ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2019-0145